Department of Urology, University of Yamanashi Graduate School of Medical Science, Chuo, Yamanashi, Japan.
Department of Urology, Fujiyoshida Municipal Medical Center, Fujiyoshida, Yamanashi, Japan.
Neurourol Urodyn. 2018 Mar;37(3):1137-1143. doi: 10.1002/nau.23436. Epub 2017 Oct 17.
We evaluated the association between lower urinary tract symptoms (LUTS) and the expression of connexin (Cx) and transient receptor potential (TRP) channel on urothelial cells non-invasively collected from voided urine in humans.
A total of 55 patients (36 males and 19 females, median age: 71 years old), who were followed up at University of Yamanashi Hospital, were enrolled in the present study. Urothelial cells were collected from voided urine of patients, and the mRNA expression of each subtype of Cxs and TRP channels was measured using quantitive real-time reverse transcription polymerase chain reaction. We then analyzed the correlation between the expression of Cxs and TRP channels and symptom scores in International Prostate Symptom Scoreand Overactive Bladder Symptom Score, in addition to Interstitial Cystitis Symptom Index (ICSI) from only interstitial cystitis (IC) patients.
Non-adjusted statistical procedure using Spearman's rank-correlation showed that there were significant correlations between the following expressions and symptom scores; (positive correlations) Cx26 versus urgency score, Cx40 versus nocturia, TRPM2 versus intermittency, TRPV1 versus urge incontinence, (negative correlation) Cx40 versus intermittency, TRPM7 versus pollakisuria. However, a multiple comparison adjustment using Bonferroni correction showed that only Cx40 had a trend of correlation with nocturia in ICSI.
The expressions of Cxs and TRP channels on urothelial cells in voided urine could be related to LUTS. Further analysis of urothelial cells in voided urine has the potential to reveal the mechanism of the LUTS and develop new markers with non-invasive methods.
我们评估了下尿路症状（LUTS）与在人类排尿时非侵入性收集的尿路上皮细胞中连接蛋白（Cx）和瞬时受体电位（TRP）通道表达之间的关联。
共纳入 55 例患者（36 名男性和 19 名女性，中位年龄：71 岁），这些患者在山梨大学医院接受随访。从患者的排尿中收集尿路上皮细胞，并使用定量实时逆转录聚合酶链反应测量每种 Cx 和 TRP 通道亚型的 mRNA 表达。然后，我们分析了 Cx 和 TRP 通道的表达与国际前列腺症状评分和膀胱过度活动症症状评分中的症状评分之间的相关性，以及仅来自间质性膀胱炎（IC）患者的间质性膀胱炎症状指数（ICSI）中的相关性。
使用 Spearman 等级相关的非调整统计程序显示，以下表达与症状评分之间存在显著相关性；（正相关）Cx26 与尿急评分，Cx40 与夜尿症，TRPM2 与间歇性，TRPV1 与急迫性尿失禁，（负相关）Cx40 与间歇性，TRPM7 与多尿症。然而，使用 Bonferroni 校正的多重比较调整显示，仅 Cx40 与 ICSI 中的夜尿症有相关性趋势。
排尿时尿路上皮细胞中 Cx 和 TRP 通道的表达可能与 LUTS 有关。进一步分析排尿时的尿路上皮细胞有可能揭示 LUTS 的机制，并开发具有非侵入性方法的新标志物。
