Uhl G R, Navia B, Douglas J
NIDA, Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224.
J Neurosci. 1988 Dec;8(12):4755-64. doi: 10.1523/JNEUROSCI.08-12-04755.1988.
Preproenkephalin and preprodynorphin mRNAs can be detected by in situ hybridization in medium-sized striatal neurons in normal rats and in rats with unilateral cerebral cortical lesions. Hybridization of 35S-labeled oligonucleotide cDNAs complementary to specific regions of each mRNA reveals that preproenkephalin-expressing neurons are more numerous than cells expressing preprodynorphin. Hybridization densities above enkephalin-positive neurons are also more than twice those noted above preprodynorphin-expressing cells. Northern analyses of mRNA extracted from the striatum are consistent with these relationships. The striatal preproenkephalin hybridization densities are decreased ipsilateral to cerebral cortical lesions; this change evolves largely between 1 and 5 d following the lesion. Striatal preproenkephalin mRNA is thus more prominent than preprodynorphin mRNA and depends on cerebral cortical inputs for its full expression.
在正常大鼠和单侧大脑皮质损伤的大鼠中，可通过原位杂交在中等大小的纹状体神经元中检测到前脑啡肽原和前强啡肽原mRNA。与每种mRNA的特定区域互补的35S标记寡核苷酸cDNA杂交显示，表达前脑啡肽原的神经元比表达前强啡肽原的细胞更多。脑啡肽阳性神经元上方的杂交密度也比前强啡肽原表达细胞上方的密度高出两倍多。从纹状体提取的mRNA的Northern分析与这些关系一致。大脑皮质损伤同侧的纹状体前脑啡肽原杂交密度降低；这种变化主要在损伤后1至5天内发生。因此，纹状体前脑啡肽原mRNA比前强啡肽原mRNA更显著，并且其充分表达依赖于大脑皮质输入。
