Smith-Brown Paula, Morrison Mark, Krause Lutz, Davies Peter S W
Children's Nutrition Research Centre, Child Health Research Centre.
The University of Queensland Diamantina Institute, Translational Research Institute, The University of Queensland, Queensland, Australia.
J Pediatr Gastroenterol Nutr. 2018 Jan;66(1):147-151. doi: 10.1097/MPG.0000000000001780.
Maturation of the gut microbiota has been shown to influence childhood growth, whereas alterations in microbiota composition are proposed to be causally related to the development of overweight and obesity. The objective of this study is to explore the association between microbiota profile, body size, and body composition in young children.
Fecal microbiota was examined by 16S rRNA gene sequencing, whereas body composition was assessed using the deuterium oxide dilution technique in a cohort of 37 well-nourished 2- to 3-year-old Australian children.
Microbiota composition (weighted UniFrac distance) was shown to be significantly associated with FFMI (fat-free mass index) z score (P = 0.027, adonis) in boys but not girls. In boys, FFMI z score was significantly correlated with the relative abundance of an OUT (Operational Taxonomic Unit) belonging to the Ruminococcaceae family (Rho = 0.822, P < 0.001, pFDR (false discovery rate adjusted P value) = 0.002, n = 18). At a FDR <0.2, FFMI z score in boys was positively associated with the relative abundance of OTU related to Dorea formicigenerans and Faecalibacterium prausnitzii and negatively correlated to an OTU related to Bacteroides cellulosilyticus.
These results suggest that previously reported associations between microbiota composition and body size may be driven by an association with fat-free mass, particularly in males.
肠道微生物群的成熟已被证明会影响儿童生长，而微生物群组成的改变被认为与超重和肥胖的发生存在因果关系。本研究的目的是探讨幼儿微生物群特征、体型和身体成分之间的关联。
对37名营养良好的2至3岁澳大利亚儿童组成的队列，通过16S rRNA基因测序检测粪便微生物群，同时使用氧化氘稀释技术评估身体成分。
微生物群组成（加权UniFrac距离）在男孩中与去脂体重指数（FFMI）z评分显著相关（P = 0.027，adonis检验），而在女孩中无此关联。在男孩中，FFMI z评分与属于瘤胃球菌科的一个可操作分类单元（OUT）的相对丰度显著相关（Rho = 0.822，P < 0.001，经错误发现率调整的P值（pFDR）= 0.002，n = 18）。在错误发现率<0.2时，男孩的FFMI z评分与与甲酸多雷亚菌和普拉梭菌相关的OTU相对丰度呈正相关，与与解纤维素拟杆菌相关的OTU呈负相关。
这些结果表明，先前报道的微生物群组成与体型之间的关联可能是由与去脂体重的关联驱动的，尤其是在男性中。
