Pereira Adriano E, Vélez Ana M, Meinke Lance J, Siegfried Blair D
Plant Genetics Research Unit, USDA/ARS.
Department of Entomology, University of Nebraska.
J Econ Entomol. 2017 Dec 5;110(6):2545-2553. doi: 10.1093/jee/tox263.
RNA interference is a powerful tool against corn rootworm. Adults and neonates of southern corn rootworm, Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae), were exposed to the LC50 of vATPase-A and Snf7 double-stranded RNAs (dsRNAs), and the effects on female fecundity, egg viability, male fitness as measured by sperm viability and mating capacity, larval recovery along with dry weight, and instar determination 10 d after exposure to dsRNA, were determined. Significant reductions were observed for a number of parameters in dsRNA-exposed rootworms relative to control treatments. Female fecundity and larval recovery were significantly reduced after exposure to both dsRNAs. In addition, larval dry weight and recovery of 2nd and 3rd instars along with dry weight for 3rd instars were significantly reduced after neonate exposure to vATPase-A dsRNA. Neither dsRNA affected male capacity to mate or sperm viability after exposure to the respective LC50s. After 10 d of feeding on untreated corn roots, neonates that survived exposure for 2 d to the vATPase-A dsRNA LC50 exhibited lower dry weight than the control. There was significant gene knockdown in adult males and females after exposure for 5 d to LC50 of vATPase-A and Snf7 dsRNAs. The parameters are discussed in terms of fitness and possible outcomes after deployment of corn hybrids expressing dsRNAs.
RNA干扰是对抗玉米根虫的一种强大工具。南方玉米根虫（Diabrotica undecimpunctata howardi Barber，鞘翅目：叶甲科）的成虫和新生幼虫被暴露于液泡型ATP酶A（vATPase-A）和液泡分选蛋白7（Snf7）双链RNA（dsRNA）的半数致死浓度（LC50）下，然后测定其对雌虫繁殖力、卵活力、通过精子活力和交配能力衡量的雄虫健康度、幼虫回收率以及干重的影响，同时还测定了暴露于dsRNA 10天后的龄期判定情况。与对照处理相比，在暴露于dsRNA的根虫中观察到许多参数有显著降低。暴露于两种dsRNA后，雌虫繁殖力和幼虫回收率均显著降低。此外，新生幼虫暴露于vATPase-A dsRNA后，幼虫干重以及二龄和三龄幼虫的回收率连同三龄幼虫的干重均显著降低。暴露于各自的LC50后，两种dsRNA均未影响雄虫的交配能力或精子活力。在以未处理的玉米根为食10天后，在vATPase-A dsRNA的LC50浓度下暴露2天仍存活的新生幼虫的干重低于对照组。在暴露于vATPase-A和Snf7 dsRNA的LC50浓度5天后，成虫雄虫和雌虫中均出现了显著的基因敲低现象。文中根据表达dsRNA的玉米杂交种的健康度和可能的结果对这些参数进行了讨论。
