DuPont Pioneer, Johnston, IA.
J Econ Entomol. 2019 May 22;112(3):1190-1196. doi: 10.1093/jee/toz029.
Western corn rootworm (Diabrotica virgifera virgifera LeConte) presents significant pest management challenges for farmers in both North America and Europe. IPD072Aa, a protein derived from Pseudomonas chlororaphis, has previously been shown to have activity against western corn rootworm. In the current study, the spectrum of activity of IPD072Aa was evaluated in controlled laboratory diet bioassays. IPD072Aa was fed at high concentrations in subchronic or chronic bioassays to 11 different insect species, representing 4 families within Coleoptera, and an additional 4 species representing four families of Lepidoptera. No adverse effects were noted in the Lepidoptera species. Within the order Coleoptera, western corn rootworm was the most sensitive species tested. A range of responses was observed within each of the four families of Coleoptera evaluated that included either no-observed effects or reduced growth, developmental delays, and/or reduced survival. These data will help inform the environmental risk assessment of genetically modified plants that express the IPD072Aa protein for western corn rootworm control.
西方玉米根萤叶甲（Diabrotica virgifera virgifera LeConte）对北美和欧洲的农民来说是一个重大的害虫管理挑战。来自假单胞菌（Pseudomonas chlororaphis）的 IPD072Aa 蛋白先前已被证明对西方玉米根萤叶甲具有活性。在当前的研究中，在控制实验室饮食生物测定中评估了 IPD072Aa 的活性谱。在亚慢性或慢性生物测定中，以高浓度喂食 11 种不同的昆虫物种，代表鞘翅目（Coleoptera）的 4 个科，以及另外 4 个代表鳞翅目（Lepidoptera）的 4 个科的物种。在鳞翅目物种中未观察到不良反应。在鞘翅目目中，西方玉米根萤叶甲是测试的最敏感物种。在所评估的四个科的每个科中都观察到了一系列反应，包括无观察到的影响或生长减少、发育延迟和/或存活率降低。这些数据将有助于为表达 IPD072Aa 蛋白控制西方玉米根萤叶甲的转基因植物的环境风险评估提供信息。
