Department of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 HZ Amsterdam, the Netherlands.
Department of Hematology, Erasmus University Medical Center, 3000 CA Rotterdam, the Netherlands.
Cell Rep. 2017 Oct 17;21(3):823-833. doi: 10.1016/j.celrep.2017.09.070.
A substantial number of human and mouse group 3 innate lymphoid cells (ILC3s) reside in secondary lymphoid organs, yet the phenotype and function of these ILC3s is incompletely understood. Here, we employed an unbiased cross-tissue transcriptomic approach to compare human ILC3s from non-inflamed lymph nodes and spleen to their phenotypic counterparts in inflamed tonsils and from circulation. These analyses revealed that, in the absence of inflammation, lymphoid organ-residing ILC3s lack transcription of cytokines associated with classical ILC3 functions. This was independent of expression of the natural cytotoxicity receptor NKp44. However, and in contrast to ILC3s from peripheral blood, lymphoid organ-residing ILC3s express activating cytokine receptors and have acquired the ability to be recruited into immune responses by inflammatory cytokines. This comprehensive cross-tissue dataset will allow for identification of functional changes in human lymphoid organ ILC3s associated with human disease.
大量的人和鼠的 3 组固有淋巴细胞（ILC3）存在于次级淋巴器官中，但这些 ILC3 的表型和功能尚不完全清楚。在这里，我们采用了一种无偏的跨组织转录组学方法，比较了非炎症性淋巴结和脾脏中的人 ILC3 与其在炎症性扁桃体和循环中的表型对应物。这些分析表明，在没有炎症的情况下，淋巴器官驻留的 ILC3 缺乏与经典 ILC3 功能相关的细胞因子的转录。这与自然细胞毒性受体 NKp44 的表达无关。然而，与外周血中的 ILC3 不同的是，淋巴器官驻留的 ILC3 表达激活细胞因子受体，并获得了通过炎症细胞因子被招募到免疫反应中的能力。这个全面的跨组织数据集将有助于识别与人类疾病相关的人类淋巴器官 ILC3 的功能变化。
