Mazzega E, de Marco A
Laboratory for Environmental and Life Sciences, University of Nova Gorica, Vipava, Slovenia.
Vet Comp Oncol. 2018 Mar;16(1):E202-E206. doi: 10.1111/vco.12359. Epub 2017 Oct 18.
Antibodies cross-reacting with homologue antigens in different species would be essential reagents for the development of comparative oncology studies. In comparison with conventional immunoglobulin Gs, recombinant nanobodies (single-domain variable regions of heavy-chain only antibodies of Camelidae origin) can be easily isolated in vitro and engineered into a variety of reagents with optimized characteristics for different research and clinical applications. We recovered an anti-human epidermal growth factor receptor 2 (anti-HER2) nanobody from a naïve llama library by direct panning on whole cells and expressed it fused to Fc and green fluorescent protein. These immunoreagents were assessed by flow cytometry and immunofluorescence with both human and canine cells overexpressing HER2 and its canine homologue dog epidermal growth factor receptor 2. The reported data illustrate the potential of using this class of antibodies in comparative oncology and suggest some development perspectives enabled by in vitro panning of pre-immune nanobody libraries.
与不同物种中的同源抗原发生交叉反应的抗体将是开展比较肿瘤学研究的重要试剂。与传统免疫球蛋白G相比，重组纳米抗体（仅来源于骆驼科动物重链抗体的单域可变区）能够在体外轻松分离，并可设计成具有不同研究和临床应用所需优化特性的多种试剂。我们通过直接在全细胞上淘选，从一只未经免疫的美洲驼文库中获得了一种抗人表皮生长因子受体2（抗HER2）纳米抗体，并将其与Fc和绿色荧光蛋白融合表达。通过流式细胞术和免疫荧光法，使用过表达HER2及其犬类同源物犬表皮生长因子受体2的人和犬细胞对这些免疫试剂进行了评估。所报道的数据说明了这类抗体在比较肿瘤学中的应用潜力，并提出了通过对免疫前纳米抗体文库进行体外淘选实现的一些发展前景。
