Brown S E, Morrison H G, Buckley S M, Shope R E, Knudson D L
Department of Entomology, Colorado State University, Fort Collins 80523.
Acta Virol. 1988 Sep;32(5):369-78.
The dsRNA profiles of the Czechoslovakian and Siberian serotypes of the Kemerovo serocomplex viruses examined were similar in agarose, while their dsRNA profiles were distinct in polyacrylamide gel. Blot hybridization studies of the Kemerovo serocomplex viruses demonstrated that the genes were highly conserved among the members within each type, but not between types. Gene reassortment in vitro was demonstrated among selected pairs of the Kemerovo serocomplex viruses by intra- and inter-typic crosses. The majority of the reassortant progeny from inter-typic crosses were single gene replacements, whereas the majority of the reassortant progeny from intra-typic crosses were multiple gene replacements suggesting that certain gene combinations were restrictive under conditions of the experiment.
所检测的克麦罗沃血清复合体病毒的捷克斯洛伐克血清型和西伯利亚血清型的双链RNA图谱在琼脂糖凝胶中相似，而在聚丙烯酰胺凝胶中则不同。对克麦罗沃血清复合体病毒的印迹杂交研究表明，基因在每种血清型内的成员间高度保守，但不同血清型之间则不然。通过型内和型间杂交，在选定的克麦罗沃血清复合体病毒对之间证明了体外基因重配。型间杂交产生的重组后代大多数是单基因替换，而型内杂交产生的重组后代大多数是多基因替换，这表明在实验条件下某些基因组合具有限制性。
