Brown S E, Morrison H G, Karabatsos N, Knudson D L
Yale Arbovirus Research Unit, Yale Department of Public Health, Yale University School of Medicine, New Haven, Connecticut 06510.
J Gen Virol. 1991 May;72 ( Pt 5):1065-72. doi: 10.1099/0022-1317-72-5-1065.
Orungo and Lebombo orbivirus isolates were examined for their intra- and intergroup genetic relatedness by blot hybridization and gene reassortment; blot hybridization was also used to examine the relatedness of selected Orungo and Lebombo isolates to known orbiviruses. Among the Orungo isolates, greater than 74% sequence similarity was shown in the majority of their genes. Gene 2 was the most divergent gene, with four unique types identified, and genes 5, 6 and 10 were variant among the isolates. Plaque reduction neutralization tests revealed at least four serotypes, a result which correlated with the hybridization data. Gene reassortment was shown between two representative Orungo isolates. Among the Lebombo isolates, two hybridization types were identified between which gene reassortment was demonstrated. Unique genes were not shown, whereas genes 2, 5 and 10 exhibited minor sequence variability. Geographic distribution correlated with relatedness among the Lebombo isolates, which was not the case among the Orungo isolates. Orungo and Lebombo viruses did not cross-hybridize or reassort their genes in vitro, in intergroup studies. In blot hybridization tests of Orungo and Lebombo isolates with known orbivirus serogroups and ungrouped orbiviruses, no strong cross-hybridization was seen. These results demonstrate that Orungo and Lebombo are distinct from each other and from other orbiviruses, and should therefore be recognized as two new Orbivirus serogroups.
通过印迹杂交和基因重配分析了奥伦戈病毒和莱邦博病毒分离株的组内和组间遗传相关性；印迹杂交还用于检测选定的奥伦戈病毒和莱邦博病毒分离株与已知环状病毒的相关性。在奥伦戈病毒分离株中，其大多数基因显示出超过74%的序列相似性。基因2是差异最大的基因，鉴定出四种独特类型，基因5、6和10在分离株中存在变异。蚀斑减少中和试验显示至少有四种血清型，这一结果与杂交数据相关。在两个代表性的奥伦戈病毒分离株之间显示出基因重配。在莱邦博病毒分离株中，鉴定出两种杂交类型，并证明了它们之间的基因重配。未发现独特基因，而基因2、5和10表现出较小的序列变异性。莱邦博病毒分离株的地理分布与其相关性相关，而奥伦戈病毒分离株则不然。在组间研究中，奥伦戈病毒和莱邦博病毒在体外不会发生交叉杂交或基因重配。在奥伦戈病毒和莱邦博病毒分离株与已知环状病毒血清群和未分组环状病毒的印迹杂交试验中，未观察到强烈的交叉杂交。这些结果表明，奥伦戈病毒和莱邦博病毒彼此不同，且与其他环状病毒不同，因此应被视为两个新的环状病毒血清群。
