Ma Junxun, Zhang Sujie, Hu Yi, Li Xiaoyan, Yuan Fang, Sun Danyang, Wang Lijie, Zhang Fan, Chen Guangying, Cui Pengfei
Department of Oncology, Chinese PLA General Hospital, Beijing, China.
Medicine (Baltimore). 2017 Oct;96(42):e7011. doi: 10.1097/MD.0000000000007011.
Ras association domain protein 10 (RASSF10) was reported to act as a prognostic indicator in various types of cancer and it was proved to be tumor suppressor gene in colorectal cancer (CRC). The purpose of this study was to evaluate the prognostic significance of RASSF10 in CRC.Quantitative real-time polymerase chain reaction was used to detect the messenger RNA (mRNA) expression while enzyme-linked immunosorbent assay was taken to measure the protein expression of RASSF10 in tumor tissues and adjacent normal tissues from 102 patients with CRC. The relationship between RASSF10 expression level and clinical characteristics of CRC patients was analyzed by chi-squared test. In addition, the association between overall survival of CRC patients and RASSF10 expression was estimated by Kaplan-Meier analysis. Cox regression analysis was used to evaluate the prognostic value of RASSF10.The expression level of RASSF10 in tumor tissues was significantly lower than that in the normal tissues both at mRNA and protein levels. Moreover, the expression level was correlated with lymph-node-metastasis and tumor-node-metastasis stage. Kaplan-Meier analysis suggested that patients with high expression level of RASSF10 had a longer overall survival than those with low level (log-rank test, P < .001). Besides, RASSF10 might be a potential biomarker in the prognosis of CRC according to cox regression analysis.The down regulated of RASSF10 is found in CRC and it may be an ideal prognostic marker.
据报道，Ras关联结构域蛋白10（RASSF10）在多种癌症中可作为预后指标，并且已被证明是结直肠癌（CRC）中的肿瘤抑制基因。本研究的目的是评估RASSF10在结直肠癌中的预后意义。采用定量实时聚合酶链反应检测102例结直肠癌患者肿瘤组织及癌旁正常组织中RASSF10的信使核糖核酸（mRNA）表达，同时采用酶联免疫吸附测定法检测其蛋白表达。采用卡方检验分析RASSF10表达水平与结直肠癌患者临床特征之间的关系。此外，采用Kaplan-Meier分析评估结直肠癌患者总生存期与RASSF10表达之间的关联。采用Cox回归分析评估RASSF10的预后价值。RASSF10在肿瘤组织中的mRNA和蛋白水平表达均显著低于正常组织。此外，其表达水平与淋巴结转移及肿瘤-淋巴结-转移分期相关。Kaplan-Meier分析表明，RASSF10高表达患者的总生存期长于低表达患者（对数秩检验，P<0.001）。此外，根据Cox回归分析，RASSF10可能是结直肠癌预后的潜在生物标志物。在结直肠癌中发现RASSF10表达下调，它可能是一个理想的预后标志物。
