Ras关联结构域蛋白10（RASSF10）启动子甲基化与胃癌生存率呈负相关。
Methylation of ras association domain protein 10 (RASSF10) promoter negative association with the survival of gastric cancer.
作者信息
Deng Jingyu, Liang Han, Ying Guoguang, Li Haixin, Xie Xingming, Yu Jun, Fan Daiming, Hao Xishan
机构信息
Department of Gastroenterology, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer Tianjin, China.
Central Laboratory, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer Tianjin, China.
出版信息
Am J Cancer Res. 2014 Nov 19;4(6):916-23. eCollection 2014.
OBJECTIVE
The present study was conducted to elucidate the prognostic prediction value of the methylation of the RASSF10 promoter in gastric cancer (GC).
METHODS
A total of 300 patients with GC revealed the methylation degrees of the DNA of the RASSF10 promoter. Methylation-specific PCR (MSP) analysis was performed to qualitatively detect the methylated degrees of the DNA of the RASSF10 promoter of 300 patients with GC. Associations between molecular, clinicopathological and survival data were analyzed.
RESULTS
The protein and mRNA expressions of RASSF10 in GC tissues were lower than those in normal gastric mucosal tissues. In the MSP analysis cohort, patients with methylated RASSF10 promoter exhibited significantly shorter median OS than those with unmethylated RASSF10 promoter (P < 0.001). Multivariate survival analysis results showed that methylated RASSF10 promoter was an independent predictor of the survival of patients with GC.
CONCLUSIONS
The methylation of the RASSF10 promoter could be applied for the clinical prediction of the prognosis of GC.
目的
本研究旨在阐明RASSF10启动子甲基化在胃癌（GC）中的预后预测价值。
方法
共300例胃癌患者检测了RASSF10启动子DNA的甲基化程度。采用甲基化特异性PCR（MSP）分析对300例胃癌患者RASSF10启动子DNA的甲基化程度进行定性检测。分析分子、临床病理和生存数据之间的关联。
结果
胃癌组织中RASSF10的蛋白和mRNA表达低于正常胃黏膜组织。在MSP分析队列中，RASSF10启动子甲基化的患者中位总生存期显著短于RASSF10启动子未甲基化的患者（P < 0.001）。多因素生存分析结果显示，RASSF10启动子甲基化是胃癌患者生存的独立预测因素。
结论
RASSF10启动子甲基化可用于胃癌预后的临床预测。
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