Dar M S
Department of Pharmacology, School of Medicine, East Carolina University, Greenville, NC 27858.
J Pharm Pharmacol. 1988 Jul;40(7):482-7. doi: 10.1111/j.2042-7158.1988.tb05282.x.
The possible biphasic effect of caffeine on acute ethanol-induced motor incoordination by rotorod evaluation was investigated in mice. Caffeine in various doses was administered intracerebroventricularly (i.c.v.) to mice implanted with permanent indwelling stainless steel guide cannulae and intraperitoneally (i.p.) to non-cannulated animals. A motor incoordinating test dose of ethanol, 2 g kg-1, was given i.p. in both cases. Caffeine less than 25 micrograms administered i.c.v., dose-dependently attenuated while 75 micrograms i.c.v. potentiated ethanol (i.p.)-induced motor incoordination. Similarly, caffeine less than 20 mg kg-1 given i.p., dose-dependently attenuated while 62.5 mg kg-1 potentiated ethanol (i.p.)-induced motor incoordination. The data obtained demonstrated that caffeine given either i.c.v. or i.p. exerted biphasic effects on ethanol-induced motor incoordination. The data also suggested that caffeine antagonized ethanol-induced motor micrograms i.c.v.; less than 20 mg kg-1 i.p.) caffeine is well known to display high affinity for adenosine binding sites. Therefore, the present investigation lends further support to our earlier suggestion that adenosine may be involved in the motor impairing effect of ethanol.
通过转棒试验评估了咖啡因对急性乙醇诱导的小鼠运动不协调的可能双相效应。对植入永久性不锈钢引导套管的小鼠进行脑室内（i.c.v.）给药不同剂量的咖啡因，对未插管的动物进行腹腔内（i.p.）给药。在两种情况下，均腹腔注射2 g kg-1的乙醇运动不协调测试剂量。脑室内注射小于25微克的咖啡因剂量依赖性地减弱了乙醇（腹腔注射）诱导的运动不协调，而脑室内注射75微克则增强了这种不协调。同样，腹腔注射小于20 mg kg-1的咖啡因剂量依赖性地减弱了乙醇（腹腔注射）诱导的运动不协调，而62.5 mg kg-1则增强了这种不协调。获得的数据表明，脑室内或腹腔内给予咖啡因对乙醇诱导的运动不协调具有双相效应。数据还表明，咖啡因拮抗乙醇诱导的运动（脑室内小于25微克；腹腔内小于20 mg kg-1）咖啡因对腺苷结合位点具有高亲和力。因此，本研究进一步支持了我们早期的观点，即腺苷可能参与了乙醇的运动损害作用。
