a Laboratory of Exercise Physiology, Faculty of Physical Education, University of Campinas, Campinas, São Paulo 13.083-851, Brazil.
c Molecular Biology Laboratory, Boldrini Children Hospital, Campinas, São Paulo 13.083-210, Brazil.
Appl Physiol Nutr Metab. 2018 Mar;43(3):240-246. doi: 10.1139/apnm-2017-0471. Epub 2017 Oct 19.
The present study aimed to compare the early metabolic response between high-load resistance exercise (HL-RE) and low-load resistance exercise with blood flow restriction (LL-BFR). Nine young, well-trained men participated in a randomized crossover design in which each subject completed LL-BFR, HL-RE, or condition control (no exercise) with a 1-week interval between them. Blood samples were taken immediately before and 5 min after the exercise sessions. Nuclear magnetic resonance spectroscopy identified and quantified 48 metabolites, 6 of which presented significant changes among the exercise protocols. The HL-RE promoted a higher increase in pyruvate, lactate, and alanine compared with the LL-BFR and the control. HL-RE and LL-BFR promoted a higher increase in succinate compared with the control; however, there was no difference between HL-RE and LL-BFR. Also, while there was no difference in acetoacetate between HL-RE and LL-BFR, a greater decrease was observed in both compared with the control. Finally, LL-BFR promoted a greater decrease in choline compared with the control. In conclusion, this study provides by metabolomics a new insight in metabolic response between LL-BFR and HL-RE by demonstrating a distinct response to some metabolites that are not commonly analyzed.
本研究旨在比较高负荷抗阻运动（HL-RE）和低负荷抗阻运动结合血流限制（LL-BFR）的早期代谢反应。9 名年轻、训练有素的男性参与了随机交叉设计的研究，每个受试者在 1 周的间隔内分别完成 LL-BFR、HL-RE 或对照条件（不运动）。在运动前后立即采集血样。核磁共振波谱鉴定并定量了 48 种代谢物，其中 6 种在运动方案之间发生了显著变化。与 LL-BFR 和对照相比，HL-RE 促进了丙酮酸、乳酸和丙氨酸更高的增加。HL-RE 和 LL-BFR 与对照相比，琥珀酸的增加更高；然而，HL-RE 和 LL-BFR 之间没有差异。此外，HL-RE 和 LL-BFR 之间的乙酰乙酸酯没有差异，但与对照相比，两者的下降幅度更大。最后，LL-BFR 与对照相比，促进了胆碱的更大下降。总之，本研究通过代谢组学提供了 LL-BFR 和 HL-RE 之间代谢反应的新见解，表明对一些通常不分析的代谢物有不同的反应。
