Universidade Federal de São Paulo (UNIFESP), Departamento de Pediatria, Disciplina de Gastroenterologia Pediátrica, São Paulo, SP, Brazil.
Universidade Federal de São Paulo (UNIFESP), Departamento de Pediatria, Disciplina de Gastroenterologia Pediátrica, São Paulo, SP, Brazil.
J Pediatr (Rio J). 2018 Sep-Oct;94(5):483-490. doi: 10.1016/j.jped.2017.09.003. Epub 2017 Oct 16.
To analyze the fecal microbiota composition of children living in an urban slum in Brazil, with or without small intestinal bacterial overgrowth, and to investigate the occurrence of stunting and anemia.
A total of 100 children were studied, aged 5-11 years, from the municipality of Osasco, São Paulo. Small intestinal bacterial overgrowth was screened through hydrogen and methane breath test with lactulose. Weight and height were measured, and the height-for-age and body mass-for-age anthropometric indexes were calculated. The occurrence of anemia was investigated by capillary hemoglobin. Analysis of bacterial phylum, genus, and species was performed by real-time polymerase chain reaction in fecal samples.
Small intestinal bacterial overgrowth was identified in 61.0% of the children. A lower mean of height-for-age Z-score ([-0.48±0.90] vs. [-0.11±0.97]; p=0.027), as well as capillary hemoglobin ([12.61±1.03g/dL] vs. [13.44±1.19g/dL]; p<0.001) was demonstrated in children with SIBO when compared with children without small intestinal bacterial overgrowth. Children with small intestinal bacterial overgrowth presented a higher frequency of Salmonella spp., when compared to those without small intestinal bacterial overgrowth (37.7% vs. 10.3%; p=0.002). Higher counts of total Eubacteria (p=0.014) and Firmicutes (p=0.038) were observed in children without small intestinal bacterial overgrowth; however, a higher count of Salmonella (p=0.002) was found in children with small intestinal bacterial overgrowth.
Children who lived in a slum and were diagnosed with small intestinal bacterial overgrowth showed lower H/A Z-scores and hemoglobin levels. Furthermore, differences were observed in the fecal microbiota of children with small intestinal bacterial overgrowth, when compared to those without it; specifically, a higher frequency and count of Salmonella, and lower counts of Firmicutes and total Eubacteria.
分析巴西城市贫民窟中有无小肠细菌过度生长的儿童的粪便微生物组成，并调查其发育迟缓与贫血的发生情况。
共纳入 100 名 5-11 岁的来自圣保罗州奥萨斯库市的儿童。通过乳果糖氢甲烷呼气试验筛查小肠细菌过度生长。测量体重和身高，并计算身高年龄别和体重年龄别人体测量学指标。通过毛细血管血红蛋白检测来调查贫血的发生情况。通过实时聚合酶链反应分析粪便样本中的细菌门、属和种。
61.0%的儿童被诊断为小肠细菌过度生长。与无小肠细菌过度生长的儿童相比，有小肠细菌过度生长的儿童的平均身高年龄别 Z 评分较低[-0.48±0.90 与-0.11±0.97;p=0.027]，毛细血管血红蛋白也较低[12.61±1.03g/dL 与 13.44±1.19g/dL;p<0.001]。与无小肠细菌过度生长的儿童相比，有小肠细菌过度生长的儿童中更常检出沙门氏菌（37.7%与 10.3%;p=0.002）。无小肠细菌过度生长的儿童中总真细菌（p=0.014）和厚壁菌门（Firmicutes）（p=0.038）的计数较高；然而，有小肠细菌过度生长的儿童中沙门氏菌的计数较高（p=0.002）。
生活在贫民窟并被诊断为小肠细菌过度生长的儿童的 H/A Z 评分和血红蛋白水平较低。此外，与无小肠细菌过度生长的儿童相比，有小肠细菌过度生长的儿童的粪便微生物群存在差异；具体而言，沙门氏菌的检出率和计数较高，而厚壁菌门和总真细菌的计数较低。
