He Jianan, Ou Qingye, Liu Chunxiao, Shi Lei, Zhao Chunzhong, Xu Yunqing, Kong Siu Kai, Loo Jacky, Li Boan, Gu Dayong
Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, 518033, PR China; Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518010, PR China.
Zhuhai Center for Chronic Disease Control, Zhuhai, 519000, PR China.
Tuberculosis (Edinb). 2017 Dec;107:73-79. doi: 10.1016/j.tube.2017.08.007. Epub 2017 Aug 24.
Tuberculosis (TB) remains a major worldwide health problem and has caused millions of deaths in the past few years. Current diagnostic methods, such as sputum smear microscopy and sputum culture, are time-consuming and cannot prevent the rapid spreading of TB during the diagnostic period. In this connection, detecting biomarkers specific to TB at molecular level in plasma of patients will provide a rapid means for diagnosis. In this study, we first evaluated the differential expression of the long non-coding RNAs (lncRNAs) in the plasma from patients with TB (TB positive), community acquired pneumonia (CAP) and healthy individuals (CG) using lncRNA microarray scanning. It was found that there were 2116 specific lncRNAs differentially expressed in the TB positive samples (1102 up-regulated and 1014 down-regulated), which accounted for 6.96% of total lncRNAs. Twelve differentially expressed lncRNAs discovered in microarray were subsequently validated by using real-time quantitative PCR (RT-qPCR). Two lncRNAs (ENST00000354432 and ENST00000427151) were further validated with more Tuberculosis samples. These results suggested the expression level of lncRNAs and the two validated lncRNAs in plasma could be the potential molecular biomarkers for the rapid diagnosis of Tuberculosis.
结核病（TB）仍然是全球主要的健康问题，在过去几年中已导致数百万人死亡。目前的诊断方法，如痰涂片显微镜检查和痰培养，耗时较长，且无法在诊断期间阻止结核病的快速传播。就此而言，在患者血浆中分子水平检测结核病特异性生物标志物将为诊断提供一种快速手段。在本研究中，我们首先使用长链非编码RNA（lncRNA）微阵列扫描评估了结核病患者（TB阳性）、社区获得性肺炎（CAP）患者和健康个体（CG）血浆中lncRNA的差异表达。结果发现，TB阳性样本中有2116种特异性lncRNA差异表达（1102种上调，1014种下调），占lncRNA总数的6.96%。随后，通过实时定量PCR（RT-qPCR）对微阵列中发现的12种差异表达lncRNA进行了验证。使用更多结核病样本进一步验证了两种lncRNA（ENST00000354432和ENST00000427151）。这些结果表明，血浆中lncRNA的表达水平以及这两种经过验证的lncRNA可能是结核病快速诊断的潜在分子生物标志物。
