Bora Kundan Singh, Kumar Akhilesh, Bisht Gajraj
Department of Pharmacognosy, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences & Research, Balawala, Dehradun, Uttarakhand, 240001, India.
Department of Pharmacognosy, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences & Research, Balawala, Dehradun, Uttarakhand, 240001, India.
J Ayurveda Integr Med. 2018 Jul-Sep;9(3):190-194. doi: 10.1016/j.jaim.2017.02.009. Epub 2017 Oct 16.
The plant Ulmus wallichiana Planch. is found in hills of Uttarakhand, India. Bark of U. wallichiana is commonly used as traditional healer for bone fracture of animals as well as human beings and also used as wound healer remedy.
The present study was designed to evaluate antimicrobial potential of various extracts of U. wallichiana bark.
Soxhlet extraction method was used for preparation of different extracts viz. petroleum ether, chloroform, ethyl acetate, ethanol and aqueous. Antioxidant activity was determined by DPPH and FRAP assay method. In vitro antimicrobial activity was evaluated using agar well diffusion method.
Ethyl acetate extract exhibited the highest significant antioxidant activity. Antibacterial activity was performed against Escherichia coli, Bacillus subtilis, Staphylococcusaureus and Pseudomonas aeruginosa. Amongst the various extracts tested, only ethyl acetate exhibited highest zone of inhibition as compared to other extracts and greater than standard drug. Chloroform extract also showed moderate zone of inhibition. Antifungal activity was evaluated against Aspergillus fumigates and Aspergillus flavus. The ethyl acetate extract showed maximum zone of inhibition as compared to other extracts. Chloroform extract showed mild antifungal activity. Chloramphenicol and nystatin were used as a positive control as antibacterial and antifungal agent respectively. Furthermore, the highest percentage of phenolic and flavonoid compounds was estimated in ethyl acetate extract.
The ethyl acetate extract of U. wallichiana showed the highest antimicrobial activity, and should be further investigated for isolating active compound(s) responsible for antimicrobial activity.
印度北阿坎德邦的山区生长着植物瓦氏榆（Ulmus wallichiana Planch.）。瓦氏榆的树皮通常被用作治疗动物和人类骨折的传统药物，也被用作伤口愈合药物。
本研究旨在评估瓦氏榆树皮不同提取物的抗菌潜力。
采用索氏提取法制备不同提取物，即石油醚提取物、氯仿提取物、乙酸乙酯提取物、乙醇提取物和水提取物。通过DPPH和FRAP分析法测定抗氧化活性。采用琼脂扩散法评估体外抗菌活性。
乙酸乙酯提取物表现出最高的显著抗氧化活性。对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌和铜绿假单胞菌进行了抗菌活性测试。在测试的各种提取物中，与其他提取物相比，只有乙酸乙酯表现出最高的抑菌圈，且大于标准药物。氯仿提取物也显示出中等抑菌圈。对烟曲霉和黄曲霉进行了抗真菌活性评估。与其他提取物相比，乙酸乙酯提取物表现出最大的抑菌圈。氯仿提取物表现出轻微的抗真菌活性。分别使用氯霉素和制霉菌素作为抗菌和抗真菌的阳性对照。此外，乙酸乙酯提取物中酚类和黄酮类化合物的含量最高。
瓦氏榆的乙酸乙酯提取物表现出最高的抗菌活性，应进一步研究以分离出具有抗菌活性的活性化合物。
