Computational Phytochemistry Lab, PG and Research Department of Botany and Microbiology, AVVM Sri Pushpam College (Autonomous) Poondi, Thanjavur (Dist), Tamil Nadu, India.
Computational Phytochemistry Lab, PG and Research Department of Botany and Microbiology, AVVM Sri Pushpam College (Autonomous) Poondi, Thanjavur (Dist), Tamil Nadu, India.
Microb Pathog. 2018 Oct;123:219-226. doi: 10.1016/j.micpath.2018.07.009. Epub 2018 Jul 19.
The aim of the present study is to investigate the antimicrobial potency of leaves from various extracts of Capparis zeylanica, Streblus asper and Tribulus terrestris were evaluated. In addition, this is the first report on MIC, MBC/MFC antimicrobial activities of above mentioned plants and also identify the phytochemical, functional groups by GC-MS and FT-IR respectively. Soxhlet extraction method was used for preparation of different extracts viz., aqueous, petroleum ether, ethyl acetate and methanol. The extracts were examined against Staphylococcus epidermidis, Enterococcus faecallis, Salmonella paratyphi, Shigella dysenteriae, Candida albicans and Mycobacterium tuberculosis by agar well diffusion method, and Minimum Inhibitory Concentratioon (MIC), Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) values were determined through micro dilution method. Phytochemical analysis of compounds was carried out by GC-MS analysis and functional groups were identified by FT-IR. Based on the outcome of our results, Ethyl acetate extract Showed significant antimicrobial activity against the tested pathogens especially, for C. albicans (40 mm) followed by ethyl acetate of S. asper against S. paratyphi (38 mm). While, the least inhibition was observed with aqueous extract of T. terrestris against S. paratyphi (10 mm). The MIC ranged from 3.21 mg/ml to 50 mg/ml and MBC/MFC 6.25 mg/ml to 50 mg/ml was recorded. Ethyl acetate extracts of almost all samples showed better activity than other extracts in inhibition growth of pathogens. Phytochemical analysis exhibited the presence of Steroids, tannins and cardiac glycosides were found only in ethyl acetate extract of C. zeylanica. Functional group of leaf extract was confirmed by FT-IR spectrum and GC-MS analysis of the ethyl acetate extract revealed the presence of 20 compounds. The results revealed that ethyl acetate extract of C. zeylanica leaves has potential activity than the other extracts as well as standard drugs (Gentamycin and Ketocozole). Hence, this plant may be recommended for further studies in isolation of active compounds and related pharmacological activities.
本研究旨在探讨鸡脚刺、三桠苦和地桃花叶的不同提取物的抗菌效力。此外，这也是首次报道上述植物的 MIC、MBC/MFC 抗菌活性，并分别通过 GC-MS 和 FT-IR 鉴定其植物化学成分和功能基团。采用索氏提取法制备不同提取物，分别为水提物、石油醚提物、乙酸乙酯提物和甲醇提物。采用琼脂孔扩散法检测提取物对表皮葡萄球菌、粪肠球菌、副伤寒沙门氏菌、痢疾志贺氏菌、白色念珠菌和结核分枝杆菌的抑制作用，并通过微量稀释法测定最小抑菌浓度（MIC）和最小杀菌/抑菌浓度（MBC/MFC）值。采用 GC-MS 分析进行化合物的植物化学成分分析，采用 FT-IR 鉴定功能基团。根据我们的研究结果，乙酸乙酯提取物对测试的病原体表现出显著的抗菌活性，特别是对白色念珠菌（40mm），其次是三桠苦的乙酸乙酯提取物对副伤寒沙门氏菌（38mm）。而地桃花的水提物对副伤寒沙门氏菌的抑制作用最小（10mm）。MIC 范围为 3.21mg/ml 至 50mg/ml，MBC/MFC 为 6.25mg/ml 至 50mg/ml。几乎所有样品的乙酸乙酯提取物在抑制病原体生长方面的活性均优于其他提取物。植物化学成分分析表明，甾体、单宁和强心苷仅存在于鸡脚刺的乙酸乙酯提取物中。叶提取物的功能基团通过 FT-IR 光谱得到确认，乙酸乙酯提取物的 GC-MS 分析显示存在 20 种化合物。结果表明，鸡脚刺叶的乙酸乙酯提取物比其他提取物以及标准药物（庆大霉素和酮康唑）具有更强的活性。因此，建议对该植物进行进一步的研究，以分离活性化合物并开展相关的药理活性研究。
