利用不同溶剂初步研究海滨山黧豆的抗菌活性。

Preliminary study on the antimicrobial activity of Enicostemma littorale using different solvents.

机构信息

Plant Growth Regulation Lab, Department of Botany, Annamalai University, Annamalainagar, Tamilnadu, India.

出版信息

Asian Pac J Trop Med. 2012 Jul;5(7):552-5. doi: 10.1016/S1995-7645(12)60097-2.

DOI:10.1016/S1995-7645(12)60097-2

PMID:22647818

Abstract

OBJECTIVE

To study the antimicrobial activity of Enicostemma littorale (E. littorale) using different solvents.

METHODS

Chloroform, methanol and acetone extracts of different parts of E. littorale (leaf, stem and root) were evaluated for antimicrobial activity using disc diffusion method against some gram-negative species such as Escherichia coli, Klebsiella pnemoniae, Pseudomonas aeruginosa, Salmonella typhi and gram-positive species Staphylococcus aureus, Bacillus cereus, Bacillus subtilis and two fugal species viz., Aspergillus fumigates and Aspergillus flavus.

RESULTS

The chloroform extracts showed the highest antibacterial activity. Among leaf, stem and root extracts, the stem extracts showed maximum antibacterial activity. All of the used extracts had no significant antifungal activity against Aspergillus fumigates and Aspergillus flavus. The chloroform stem extract showed highest activity (about 20 mm inhibition zone) against Bacillus subtilis (at 500 mg/mL) followed by the methanolic stem extract which showed highest activity against the same organism. The lowest antibacterial activity was observed by the acetone leaf extract (about 8 mm inhibition zone) against Escherichia coli.

CONCLUSION

The findings of the study indicate littorale could also be a new source for antibiotics discovery.

摘要

目的

使用不同溶剂研究节节草（Enicostemma littorale）的抗菌活性。

方法

采用纸片扩散法，评估节节草叶、茎和根的氯仿、甲醇和丙酮提取物对一些革兰氏阴性菌（如大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌、伤寒沙门氏菌）和革兰氏阳性菌（金黄色葡萄球菌、蜡状芽孢杆菌、枯草芽孢杆菌）的抗菌活性。

结果

氯仿提取物表现出最高的抗菌活性。在叶、茎和根提取物中，茎提取物表现出最大的抗菌活性。所有使用的提取物对烟曲霉和黄曲霉均无明显的抗真菌活性。氯仿茎提取物对枯草芽孢杆菌（500mg/mL）表现出最高的活性（约 20mm 抑菌圈），其次是甲醇茎提取物，对同一菌株表现出最高的活性。丙酮叶提取物对大肠杆菌的抗菌活性最低（约 8mm 抑菌圈）。