Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, PR China.
College of Agriculture and Forestry Science and Technology, Hebei North University, Zhangjiakou, 075000, PR China.
Int J Biol Macromol. 2018 Feb;107(Pt B):2217-2223. doi: 10.1016/j.ijbiomac.2017.10.100. Epub 2017 Oct 16.
The aim of this study was to optimize the extraction process of polysaccharides from the fruiting bodies of Lentinus edodes and investigate its anti-hepatitis B virus activity. The extracting parameters including ultrasonic power (240-320W), extraction temperature (40-60°C) and extraction time (15-25min) was optimized by using three-variable-three-level Box-Behnken design based on the single-factor experiments. Data analysis results showed that the optimal conditions for extracting LEPs were an extraction temperature of 45°C, extraction time of 21min and ultrasonic power of 290W. Under these optimal conditions, the experimental yield of LEPs was 9.75%, a 1.62-fold increase compared with conventional heat water extraction (HWE). In addition, crude polysaccharides were purified to obtain two fractions (LEP-1 and LEP-2). Chemical analysis showed that these components were rich in glucose, arabinose and mannose. Furthermore, HepG2.2.15 cells were used as in vitro models to evaluate their anti-hepatitis B virus (HBV) activity. The results suggest that LEPs possesses potent anti-HBV activity in vitro.
本研究旨在优化香菇子实体多糖的提取工艺，并考察其抗乙型肝炎病毒（HBV）活性。通过单因素实验，采用三因素三水平 Box-Behnken 设计优化超声功率（240-320W）、提取温度（40-60°C）和提取时间（15-25min）等提取参数。数据分析结果表明，提取 LEPs 的最佳条件为提取温度 45°C、提取时间 21min 和超声功率 290W。在最佳条件下，LEPs 的实验产率为 9.75%，比传统热水提取（HWE）提高了 1.62 倍。此外，粗多糖经纯化得到两种组分（LEP-1 和 LEP-2）。化学分析表明，这些成分富含葡萄糖、阿拉伯糖和甘露糖。此外，还利用 HepG2.2.15 细胞作为体外模型评价其抗 HBV 活性。结果表明，LEPs 具有体外抗 HBV 活性。
