亚临界水强化深共晶溶剂提取及其抗氧化活性多糖。

Subcritical Water Enhanced with Deep Eutectic Solvent for Extracting Polysaccharides from and Their Antioxidant Activities.

机构信息

College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.

School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.

出版信息

Molecules. 2022 Jun 4;27(11):3612. doi: 10.3390/molecules27113612.

In the present study, subcritical water extraction (SWE) assisted with deep eutectic solvent (DES) is used to extract polysaccharides (LEP). In addition, the antioxidant activity of the polysaccharide samples was also investigated. Based on a single factor test and response surface test, the optimal extraction factors were a liquid-solid solvent of 40:1 mL/g, extraction temperature of 147.23 °C, water content of 39.76% and extraction time of 17.58 min. Under these extraction conditions, the yield of LEP was 6.26 ± 0.08%. Compared with the SWE and hot water extraction (HWE), it improved by 19.24% and 17.01%, respectively. In addition, the results of monosaccharide composition, molecular weight, FT-IR, UV and SEM confirmed that the extracts had the features of polysaccharides. Interestingly, the polysaccharides obtained with the SWE assisted with the DES procedure showed a higher DPPH scavenging activity, hydroxyl radical scavenging activity and hydrogen peroxide scavenging activity, which indicated that the polysaccharides with this method had a stronger antioxidant activity. These findings demonstrated that the SWE-assisted DES is a strong method to obtain polysaccharides from for food, biopharmaceutical and other industrial production.

在本研究中，采用亚临界水萃取（SWE）辅助深共晶溶剂（DES）从中提取多糖（LEP）。此外，还研究了多糖样品的抗氧化活性。通过单因素试验和响应面试验，确定了最佳的提取条件为：液固比为 40:1 mL/g、提取温度为 147.23°C、含水量为 39.76%、提取时间为 17.58 min。在此提取条件下，LEP 的产率为 6.26±0.08%。与 SWE 和热水提取（HWE）相比，分别提高了 19.24%和 17.01%。此外，单糖组成、分子量、FT-IR、UV 和 SEM 的结果证实了提取物具有多糖的特征。有趣的是，用 SWE 辅助 DES 得到的多糖具有更高的 DPPH 清除活性、羟基自由基清除活性和过氧化氢清除活性，这表明用这种方法得到的多糖具有更强的抗氧化活性。这些发现表明，SWE 辅助 DES 是从中提取多糖的一种强有力的方法，可用于食品、生物制药和其他工业生产。