Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Science, Southwest University, Chongqing, 400715, People's Republic of China.
Arch Environ Contam Toxicol. 2018 May;74(4):515-526. doi: 10.1007/s00244-017-0454-1. Epub 2017 Oct 19.
To investigate the effect of bisphenol A (BPA) on Cylindrospermopsis raciborskii (Cyanobacteria) and Scenedesmus quadricauda (Chlorophyta), we grew the two species at BPA concentrations of 0, 0.1, 1, 2, 5, 10, and 20 mg/L and examined their growth, lipid peroxidation, antioxidant enzyme activity, and chlorophyll a fluorescence. The 96-h EC values (effective concentration causing 50% growth inhibition) for BPA in C. raciborskii and S. quadricauda were 9.663 ± 0.047, and 13.233 ± 0.069 mg/L, respectively. A significant reduction in chlorophyll a concentration was found in C. raciborskii and S. quadricauda when BPA concentrations were greater than 1 and 2 mg/L, respectively. Furthermore, F /F , ΔF/F ', and qP decreased significantly at 10 mg/L BPA in C. raciborskii but started to decrease at 10 mg/L in S. quadricauda. The changes in chlorophyll fluorescence parameters (α, rETR) that were obtained from the rapid light response curves of both algae species showed similar responses to F /F , ΔF/F ', and qP under BPA-induced stress. Values for all of the chlorophyll fluorescence parameters in S. quadricauda were higher than in C. raciborskii; however, the nonphotochemical quenching measured in C. raciborskii was considerably higher than it was in S. quadricauda. In addition, lipid peroxidation (determined as MDA content) and antioxidant enzyme activities (SOD and CAT) increased in both species as the BPA concentration increased. These results suggest that C. raciborskii is more sensitive to the effects of BPA than S. quadricauda and that photosystem II might be a target for the activity of BPA in vivo.
为了研究双酚 A（BPA）对水华鱼腥藻（蓝藻）和四尾栅藻（绿藻）的影响，我们在 BPA 浓度为 0、0.1、1、2、5、10 和 20mg/L 的条件下培养这两种藻，并检测了它们的生长情况、脂质过氧化、抗氧化酶活性和叶绿素 a 荧光。BPA 对水华鱼腥藻和四尾栅藻的 96 小时 EC 值（引起 50%生长抑制的有效浓度）分别为 9.663±0.047 和 13.233±0.069mg/L。当 BPA 浓度分别大于 1 和 2mg/L 时，水华鱼腥藻和四尾栅藻的叶绿素 a 浓度显著降低。此外，当 BPA 浓度为 10mg/L 时，水华鱼腥藻的 F / F 、ΔF/F '和 qP 显著降低，但四尾栅藻的 F / F 、ΔF/F '和 qP 开始在 10mg/L 时降低。两种藻类的快速光响应曲线获得的叶绿素荧光参数（α、rETR）的变化对 F / F 、ΔF/F '和 qP 表现出相似的响应，在 BPA 诱导的胁迫下。四尾栅藻的所有叶绿素荧光参数值均高于水华鱼腥藻；然而，水华鱼腥藻的非光化学猝灭值明显高于四尾栅藻。此外，随着 BPA 浓度的增加，两种藻类的脂质过氧化（用 MDA 含量表示）和抗氧化酶活性（SOD 和 CAT）均增加。这些结果表明，水华鱼腥藻比四尾栅藻对 BPA 的影响更为敏感，并且体内的 BPA 可能作用于光系统 II。
