Crepel F, Krupa M
Laboratoire de Neurobiologie et Neuropharmacologie du Développement, Université Paris-Sud, Orsay, France.
Brain Res. 1988 Aug 23;458(2):397-401. doi: 10.1016/0006-8993(88)90486-6.
In immature rat cerebellar slices in vitro, a long term depression (LTD) of the responses of Purkinje cells (PCs) to L-glutamate (Glu) was achieved in 30% of the recorded cells by simultaneous stimulation of the neurones by Glu and by climbing fibres (CFs). This effect was not observed for L-aspartate (Asp)-induced responses. Similarly, selective LTD of Glu-induced responses was obtained in 22% of the cells by pairing Glu applications with direct stimulation of the cells which elicited calcium spikes in these neurones. Finally, bath application of phorbol esters also induced a selective LTD of Glu-induced responses in all cells tested. These results suggest that protein kinase C is involved in cerebellar synaptic plasticity.
在体外培养的未成熟大鼠小脑切片中，通过同时用L-谷氨酸（Glu）和攀缘纤维（CFs）刺激神经元，在30%的记录细胞中实现了浦肯野细胞（PCs）对L-谷氨酸（Glu）反应的长时程抑制（LTD）。L-天冬氨酸（Asp）诱导的反应未观察到这种效应。同样，通过将Glu应用与直接刺激能在这些神经元中引发钙峰的细胞配对，在22%的细胞中获得了Glu诱导反应的选择性LTD。最后，在所有测试细胞中，浴用佛波酯也诱导了Glu诱导反应的选择性LTD。这些结果表明蛋白激酶C参与了小脑突触可塑性。
