Fazeli M S, Errington M L, Dolphin A C, Bliss T V
Department of Pharmacology and Clinical Pharmacology, St. George's Hospital Medical School, London, U.K.
Brain Res. 1988 Nov 8;473(1):51-9. doi: 10.1016/0006-8993(88)90314-9.
Changes in protein content of push-pull cannula perfusates from the dentate gyrus of anaesthetized rats were analyzed before and after the induction of long-term potentiation (LTP). LTP, induced by either high-frequency stimulation of the perforant path or raising the extracellular calcium concentration, was associated with increases in the protein content of the perfusates. Tetanically induced LTP was accompanied with a large but delayed increase (apparent in the second hour after the stimulation) in protein efflux. In contrast, when LTP was induced by the elevation of extracellular calcium concentration, a smaller but more immediate increase in protein efflux was observed. When 5-D-aminophosphonovalerate was used to block the induction of LTP, no increase was observed in either case. These results indicate that LTP in the dentate gyrus is accompanied by an increase in the efflux of proteins into push-pull cannula perfusates. The possible origins of these proteins and their role in LTP are discussed.
在麻醉大鼠齿状回的推挽式套管灌注液中，分析了长时程增强（LTP）诱导前后蛋白质含量的变化。通过对穿通通路进行高频刺激或提高细胞外钙浓度诱导的LTP，与灌注液中蛋白质含量的增加有关。强直诱导的LTP伴随着蛋白质外流的大幅但延迟增加（在刺激后第二小时明显）。相比之下，当通过提高细胞外钙浓度诱导LTP时，观察到蛋白质外流的增加较小但更直接。当使用5-D-氨基磷酸戊酸来阻断LTP的诱导时，在两种情况下均未观察到增加。这些结果表明，齿状回中的LTP伴随着蛋白质向推挽式套管灌注液中外流的增加。讨论了这些蛋白质的可能来源及其在LTP中的作用。
