Department of Medical Science & Education, Jilin Province People's Hospital, Changchun 130021, PR China.
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Feb 15;191:290-295. doi: 10.1016/j.saa.2017.10.039. Epub 2017 Oct 12.
Herein we report a novel polydopamine-silver nanoparticle-polydopamine-gold (PDA-AgNPs-PDA-Au) film based surface plasmon resonance (SPR) biosensor for horse IgG detection. The PDA-AgNPs-PDA-Au film sensing platform was built on Au-film via layer-by-layer self-assembly. Ag ion was reduced in situ to AgNPs in presence of PDA. The top PDA layer can prevent AgNPs from being oxidized and connect with antibody via Schiff alkali reaction directly. The morphology and thickness of the modified gold film were characterized using scanning electron microscope and Talystep. Experimental results show that the PDA-AgNPs-PDA-Au film sensing platform is stable, regenerative and sensitive for horse IgG detection. The detection limit of horse IgG obtained with the present biosensor is 0.625μgmL, which is 2-fold and 4-fold lower than that obtained with biosensor based on PDA modified Au film and conventional biosensor based on MPA, respectively. Furthermore, when challenged to real serum samples, our sensor exhibited excellent specificity to horse IgG, suggesting its potential for industrial application.
我们在此报告了一种新型的基于聚多巴胺-银纳米粒子-聚多巴胺-金（PDA-AgNPs-PDA-Au）膜的表面等离子体共振（SPR）生物传感器，用于马 IgG 的检测。该 PDA-AgNPs-PDA-Au 膜传感平台通过逐层自组装构建在 Au 膜上。在 PDA 的存在下，Ag 离子被原位还原为 AgNPs。顶部的 PDA 层可以防止 AgNPs 被氧化，并通过席夫碱反应直接与抗体连接。使用扫描电子显微镜和 Talystep 对修饰后的金膜的形貌和厚度进行了表征。实验结果表明，PDA-AgNPs-PDA-Au 膜传感平台稳定、可再生且对马 IgG 的检测具有敏感性。本生物传感器检测马 IgG 的检测限为 0.625μgmL，分别比基于 PDA 修饰的 Au 膜的生物传感器和基于 MPA 的传统生物传感器获得的检测限低 2 倍和 4 倍。此外，当用实际的血清样品进行测试时，我们的传感器对马 IgG 表现出优异的特异性，表明其在工业应用方面的潜力。
