Multi-layer optical fiber surface plasmon resonance biosensor based on a sandwich structure of polydopamine-MoSe@Au nanoparticles-polydopamine.

An all-optical fiber multi-layer surface plasmon resonance (SPR) biosensor based on a sandwich structure of polydopamine-MoSe@Au nanoparticles-polydopamine (PDA-MoSe@AuNPs-PDA) was designed for the detection of specific immunoreactions. By optimizing the multi-layer structure and the ratio of MoSe: AuNPs, a sensitivity of 5117.59 nm/RIU has been obtained, which is more than double that of the only Au-filmed optical fiber SPR sensor. A large surface area was produced by integrating the MoSe primitive unit cell and the AuNPs into a hybrid plasmonic nanostructure of MoSe@AuNPs, leading to optical fiber SPR signal amplification. The nanostructure of MoSe@AuNPs was surrounded by the PDA layer to guarantee the efficient immobilization of the protein molecules on the optical fiber by strong covalent bond. This biosensor achieved a detection limit of 54.05 ng/mL for detecting the goat-anti-rabbit IgG, which demonstrated enhancements of 12.1%, 23.3% and 184.6% in comparison with three reported SPR biosensors decorated with PDA-AuNPs-PDA, PDA and Cysteamine-MoSe@AuNPs-Cysteamine nanostructure, respectively. This biosensor achieved favorable selectivity and outstanding sensitivity compared with the reported SPR immuno-sensors, which will provide a miniaturized, rapid-response and label-free optical fiber bio-sensing platform for clinical diagnosis in the future.