Cui Aili, Jin Zhehu, Gao Zhonggao, Jin Mingji, Zhu Lianhua, Li Lianhua, Jin Chenglong, An Yinghua
1 Department of Dermatology, Yanbian University Hospital, Yanji, Jilin Province, P.R. China.
2 Department of Pharmaceutics, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P.R. China.
Tumour Biol. 2017 May;39(5):1010428317701640. doi: 10.1177/1010428317701640.
Accumulating evidence indicated that aberrantly expressed microRNAs play critical roles in the initiation and progression of human cancers. However, the underlying functions of miR-493 in human melanoma remains unknown. Here, our study found that miR-493 expression was downregulated in human melanoma tissues and cells. Overexpression of miR-493 suppressed cell proliferation and cell cycle in human melanoma cell line A375. IRS4 was defined as a target for downregulation by miR-493 and was confirmed by luciferase assay. We also found that knockdown of IRS4 counteracted the proliferation promotion by miR-493 inhibitor. In summary, these results demonstrated that miR-493 acts as a tumor suppressor and inhibits cell proliferation and cell cycle in human melanoma by directly targeting IRS4.
越来越多的证据表明，异常表达的微小RNA在人类癌症的发生和发展中起着关键作用。然而，miR-493在人类黑色素瘤中的潜在功能仍不清楚。在此，我们的研究发现miR-493在人类黑色素瘤组织和细胞中表达下调。miR-493的过表达抑制了人类黑色素瘤细胞系A375的细胞增殖和细胞周期。IRS4被确定为miR-493下调的靶标，并通过荧光素酶测定得到证实。我们还发现，敲低IRS4可抵消miR-493抑制剂对增殖的促进作用。总之，这些结果表明，miR-493作为一种肿瘤抑制因子，通过直接靶向IRS4抑制人类黑色素瘤的细胞增殖和细胞周期。
