State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Shizishan Street, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Shizishan Street, Wuhan 430070, China.
Bioresour Technol. 2018 Feb;249:354-360. doi: 10.1016/j.biortech.2017.09.156. Epub 2017 Oct 3.
To synthesis biodiesel from palm oil in one-time addition of methanol and solvent-free medium using CBD fused with C-terminal of lipase from G. stearothermophilus (GSlip-CBD) was immobilized onto magnetic cellulose nanosphere (MCNS). The immobilized matrix traits were preconceived by FT-IR, TEM and XRD. Perceptible biodiesel yield 98 and 73% was synthesized by GSlip-CBD-MCNS in 4 h and GSlip-MCNS in 6 h under the optimized conditions of oil:methanol ratio (1:3.5), temperature (55 and 50 °C) and enzyme loading (15 U). Intriguingly, the operational stability of GSlip-CBD-MCNS was an easily attainable owing to the magnetic properties and could be reused up to 8th and19th cycles with 94 and 45% of biodiesel yield respectively, compared to GSlip-MCNS. Thus GSlip-CBD-MCNS could be a potential biocatalyst for higher yield of biodiesel and reusability in one step addition of methanol.
使用 CBD 融合嗜热脂肪地芽孢杆菌脂肪酶的 C 端（GSlip-CBD）固定在磁性纤维素纳米球（MCNS）上，一次性添加甲醇和无溶剂介质从棕榈油中合成生物柴油。通过 FT-IR、TEM 和 XRD 预先设想了固定基质的特性。在优化的油：甲醇比（1:3.5）、温度（55 和 50°C）和酶加载量（15 U）条件下，GSlip-CBD-MCNS 在 4 h 内合成了 98%和 73%的可观生物柴油产率，而 GSlip-MCNS 在 6 h 内合成了 98%和 73%的可观生物柴油产率。有趣的是，由于磁性，GSlip-CBD-MCNS 的操作稳定性很容易实现，并且可以重复使用 8 次和 19 次，生物柴油产率分别为 94%和 45%，而 GSlip-MCNS 则为 94%和 45%。因此，GSlip-CBD-MCNS 可以作为一种潜在的生物催化剂，用于在一步添加甲醇的情况下提高生物柴油的产量和可重复使用性。
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