de Menezes Luiz Henrique Sales, do Espírito Santo Eliezer Luz, Dos Santos Marta Maria Oliveira, de Carvalho Tavares Iasnaia Maria, Mendes Adriano Aguiar, Franco Marcelo, de Oliveira Julieta Rangel
Department of Exact and Technological Sciences, State University of Santa Cruz (UESC), Ilhéus, BA, 45654-370, Brazil.
Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, AL, 57072-900, Brazil.
Biotechnol Lett. 2022 Jan;44(1):89-99. doi: 10.1007/s10529-021-03196-w. Epub 2021 Nov 5.
To immobilize Candida rugosa lipase in Accurel MP 1000 (CRL-AMP) by physical adsorption in organic medium and apply in the synthesis of wax esters dodecanoyl octadecanoate 1 and hexadecanoyl octadecanoate 2 in a heptane medium, as well as evaluating the stability and recyclability of CRL-AMP in six reaction cycles.
The specific activity (A) for CRL-AMP was 200 ± 20 U mg. Its catalytic activity was 1300 ± 100 U g. CRL-AMP was used in the synthesis of esters in heptane medium with a 1:1 acid:alcohol molar ratio at 45 °C and 200 rpm. In synthesis 1, conversion was 62.5 ± 3.9% in 30 min at 10% m v and 56.9 ± 2.8% in 54 min at 5% m v; while in synthesis 2, conversion was 79.0 ± 3.9% in 24 min at 10% m v, and 46.0 ± 2.4% in 54 min at 5% m v. Reuse tests after six consecutive cycles of reaction showed that the biocatalyst retained approximately 50% of its original activity for both reaction systems.
CRL-AMP showed a high potential in the production of wax esters, since it started from low enzymatic load and high specific activities and conversions were obtained, in addition to allowing an increase in stability and recyclability of the prepared biocatalyst.
通过在有机介质中物理吸附将皱褶假丝酵母脂肪酶固定在Accurel MP 1000（CRL-AMP）中，并应用于在庚烷介质中合成蜡酯十二烷酰十八烷酸酯1和十六烷酰十八烷酸酯2，以及评估CRL-AMP在六个反应循环中的稳定性和可回收性。
CRL-AMP的比活性（A）为200±20 U mg。其催化活性为1300±100 U g。CRL-AMP用于在庚烷介质中以1:1的酸:醇摩尔比、45℃和200 rpm合成酯。在合成1中，在10% m v时30分钟内转化率为62.5±3.9%，在5% m v时54分钟内转化率为56.9±2.8%；而在合成2中,在10% m v时24分钟内转化率为79.0±3.9%，在5% m v时54分钟内转化率为46.0±2.4%。连续六个反应循环后的重复使用测试表明，对于两个反应体系，生物催化剂均保留了其原始活性的约50%。
CRL-AMP在蜡酯生产中显示出很高的潜力，因为它起始酶负载量低，具有高比活性和转化率，此外还能提高所制备生物催化剂的稳定性和可回收性。
