Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.
Colloids Surf B Biointerfaces. 2020 Apr;188:110812. doi: 10.1016/j.colsurfb.2020.110812. Epub 2020 Jan 20.
Herein, thermophilic lipase QLM from Alcaligenes sp. has been successfully immobilized in bio-based metal-organic frameworks (MOFs) through biomimetic mineralization, using zinc acetate and adenine as metal ion and organic ligand, respectively. The morphology and structure of lipase@Bio-MOF was systematically characterized by scanning electron microcopy (SEM), transmission electron microcopy (TEM), powder X-ray diffraction (PXRD) and Fourier transform infrared spectra (FT-IR). The enzyme loading in immobilized enzyme was measured to be 15.9 % by thermogravimetric analysis (TGA). Further, it was demonstrated to possess favorable catalytic activity and stability under high temperature and alkaline conditions and in the presence of metal ions, using the hydrolysis of p-nitrophenyl caprylate as a model. Finally, the immobilized enzyme was successfully applied in the preparation of biodiesel through the trans-esterification of sunflower oil with methanol, obtaining a conversion of >60 % at a high oil/methanol ratio of 8:1. Meanwhile, it showed excellent recyclability during the biodiesel production, and no changes of morphology and crystal structure were observed after being used for 3 cycles. Overall, the immobilized lipase in bio-based MOFs provided an economically and environmentally viable biocatalyst for the synthesis of biodiesel.
在此，通过仿生矿化，使用醋酸锌和腺嘌呤分别作为金属离子和有机配体，成功地将嗜热脂肪酶 QLM 固定在基于生物的金属有机骨架（MOFs）中。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、粉末 X 射线衍射（PXRD）和傅里叶变换红外光谱（FT-IR）对脂肪酶@Bio-MOF 的形态和结构进行了系统的表征。通过热重分析（TGA）测量固定化酶中的酶载量为 15.9%。进一步，通过使用 p-硝基苯辛酸酯的水解作为模型，证明了其在高温和碱性条件下以及存在金属离子时具有良好的催化活性和稳定性。最后，通过甲醇与葵花油的酯交换反应，将固定化酶成功应用于生物柴油的制备中，在高油/甲醇比为 8:1 的情况下，转化率>60%。同时，在生物柴油生产过程中表现出优异的可回收性，使用 3 次后未观察到形态和晶体结构的变化。总的来说，基于生物的 MOFs 中的固定化脂肪酶为生物柴油的合成提供了一种经济环保的可行的生物催化剂。
