Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu/SP, 18618681, Brazil.
Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, São Paulo State University, Botucatu/SP, 18618681, Brazil.
J Dairy Sci. 2018 Jan;101(1):581-583. doi: 10.3168/jds.2017-13347. Epub 2017 Oct 18.
An acute case of clinical mastitis in a Holstein cow from second lactation is reported here. A milk sample from the affected quarter was cultured on 5% bovine blood agar and incubated at 37°C for 72 h. After 24 h of incubation, numerous colonies of yeast were observed: the Candida characteristic was not detected by CHROMagar Candida (Difco, Franklin Lakes, NJ). The DNA extraction of the isolate was performed, and DNA was subjected to amplification and sequencing of the D1/D2 region of the large subunit rRNA gene. The sequences were aligned using Mega 7.0 and used for searching GenBank by BLASTn (Basic Local Alignment Search Tool for nucleotides), revealing 98% of identity with Cyberlindnera rhodanensis. To date, this is the first report of this yeast associated with clinical bovine mastitis.
现报告一例荷斯坦奶牛处于泌乳二期的急性临床乳腺炎病例。从受影响的乳区采集奶样，接种于 5%牛血琼脂平板，在 37°C 下孵育 72 小时。孵育 24 小时后，观察到大量酵母样菌落：CHROMagar Candida（Difco，新泽西州富兰克林湖）未检测到念珠菌特征。对分离株进行 DNA 提取，对大亚基 rRNA 基因的 D1/D2 区进行 DNA 扩增和测序。使用 Mega 7.0 进行序列比对，并使用 BLASTn（核苷酸基本局部比对搜索工具）在 GenBank 中进行搜索，与 Cyberlindnera rhodanensis 的同源性为 98%。迄今为止，这是首例与奶牛临床乳腺炎相关的该酵母的报告。
