Kay H D, Fagnani R, Bonnard G D
Int J Cancer. 1979 Aug;24(2):141-50. doi: 10.1002/ijc.2910240204.
When Fc receptors (FcR) on normal human peripheral blood lymphocytes were induced to modulate by overnight (18 h) incubation in the presence of soluble or particulate immune complexes, the natural killer (NK) activity of the effector lymphocyte suspension, as measured against the K562 erythroleukemia cell line, was significantly, but only partially, inhibited. The NK activity which remained was always strong, and was not significantly inhibited by inclusion of antigen-antibody complexes in the cytotoxicity assay, nor was it further depleted by adsorbing the modulated cells on plastic surfaces coated with immobilized antigen-antibody complexes. Antibody-dependent cell-mediated cytotoxicity (ADCC) against rabbit antibody-sensitized Chang liver cells was totally abrogated following the modulation process, and could not be restored by exposure of modulated effector cells to trypsin, indicating that the FcR had actually been shed and were not merely being blocked with immune complexes. Although freshly isolated peripheral blood lymphocytes active in natural (or "spontaneous") cytotoxicity have been shown to bear FcR, our data indicate that NK activity against the K562 cell line can be effectively mediated by NK cells which have lost their FcR. This supports the concept that NK activity against K562 is independent of FcR, and, therefore, of IgG.
当正常人外周血淋巴细胞上的Fc受体（FcR）在可溶性或颗粒性免疫复合物存在的情况下经过过夜（18小时）孵育被诱导调节时，针对K562红白血病细胞系测定的效应淋巴细胞悬液的自然杀伤（NK）活性受到显著但只是部分的抑制。剩余的NK活性始终很强，在细胞毒性测定中加入抗原 - 抗体复合物对其没有显著抑制作用，用固定化抗原 - 抗体复合物包被的塑料表面吸附经调节的细胞也不会使其进一步减少。在调节过程之后，针对兔抗体致敏的Chang肝细胞的抗体依赖性细胞介导的细胞毒性（ADCC）完全被消除，并且经调节的效应细胞暴露于胰蛋白酶后也无法恢复，这表明FcR实际上已经脱落，而不仅仅是被免疫复合物阻断。尽管已经证明在自然（或“自发”）细胞毒性中具有活性的新鲜分离的外周血淋巴细胞带有FcR，但我们的数据表明，针对K562细胞系的NK活性可以由已经失去其FcR的NK细胞有效介导。这支持了针对K562的NK活性独立于FcR，因此也独立于IgG的概念。
