a Laboratoire de l'Intégration du Matériau au Système, Centre National de la Recherche Scientifique (CNRS) UMR 5218, Talence, France.
b Université de Bordeaux, Talence, France.
Radiat Res. 2018 Jan;189(1):95-103. doi: 10.1667/RR14877.1. Epub 2017 Oct 23.
The existence of effects of radiofrequency field exposure at environmental levels on living tissues and organisms remains controversial, in particular regarding potential "nonthermal" effects produced in the absence of temperature elevation. Therefore, we investigated whether TRPV1, one of the most studied thermosensitive channels, can be activated by the heat produced by radiofrequency fields and by some specific nonthermal interaction with the fields. We have recently shown that TRPV1 activation can be assessed in real-time on live cells using the bioluminescence resonance energy transfer technique. Taking advantage of this innovative assay, we monitored TRPV1 thermal and chemical modes of activation under radiofrequency exposure at 1800 MHz using different signals (CW, GSM, UMTS, LTE, Wi-Fi and WiMAX) at specific absorption rates between 8 and 32 W/kg. We showed that, as expected, TRPV1 channels were activated by the heat produced by radiofrequency field exposure of transiently-transfected HEK293T cells, but found no evidence of TRPV1 activation in the absence of temperature elevation under radiofrequency field exposure. There was no evidence either that, at fixed temperature, radiofrequency exposure altered the maximal efficacy of the agonist Capsaicin to activate TRPV1.
射频场在环境水平下对活体组织和生物的影响仍然存在争议，特别是在没有温度升高的情况下产生的潜在“非热”效应。因此，我们研究了 TRPV1（研究最多的热敏通道之一）是否可以被射频场产生的热量以及与场的某些特定非热相互作用激活。我们最近表明，可以使用生物发光共振能量转移技术实时评估活细胞中的 TRPV1 激活。利用这项创新的测定法，我们在 1800 MHz 下以不同的信号（CW、GSM、UMTS、LTE、Wi-Fi 和 WiMAX）以特定吸收率 8 至 32 W/kg 监测了 TRPV1 在射频暴露下的热和化学激活模式。我们表明，正如预期的那样，TRPV1 通道被射频场短暂转染的 HEK293T 细胞暴露产生的热量激活，但在没有温度升高的情况下没有证据表明射频场暴露下 TRPV1 的激活。也没有证据表明，在固定温度下，射频暴露会改变激动剂辣椒素激活 TRPV1 的最大功效。
