IMS Laboratory, CNRS, UMR 5218, Université de Bordeaux, Talence, France.
Limoges University, CNRS, XLIM, UMR 7252, Limoges, France.
Int J Radiat Biol. 2020 Jun;96(6):836-843. doi: 10.1080/09553002.2020.1730016. Epub 2020 Mar 4.
The present study was conducted to re-evaluate the effect of low-level 1800 MHz RF signals on RAS/MAPK activation in live cells. Using Bioluminescence Resonance Energy Transfer technique (BRET), we assessed the effect of Continuous wave (CW) and Global System for Mobile (GSM)-modulated 1800 MHz signals (up to 2 W/kg) on ERK and RAS kinases' activity in live HuH7 cells. We found that radiofrequency field (RF) exposure for 24 h altered neither basal level of RAS and ERK activation nor the potency of phorbol-12-myristate-13-acetate (PMA) to activate RAS and ERK kinases. However, we found that exposure to GSM-modulated 1800 MHz signals at 2 W/kg decreased the PMA maximal efficacy to activate both RAS and ERK kinases' activity. Exposure with CW 1800 MHz signal at 2 W/kg only decreased maximal efficacy of PMA to activate ERK but not RAS. No effects of RF exposure at 0.5 W/kg was observed on maximal efficacy of PMA to activate either RAS or ERK whatever the signal used. Our results indicate that RF exposure decreases the efficiency of the cascade of events, which, from the binding of PMA to its receptor(s), leads to the activation of RAS and ERK kinases.
本研究旨在重新评估低水平 1800MHz RF 信号对活细胞中 RAS/MAPK 激活的影响。使用生物发光共振能量转移技术（BRET），我们评估了连续波（CW）和全球移动通信系统（GSM）调制的 1800MHz 信号（高达 2W/kg）对活 HuH7 细胞中 ERK 和 RAS 激酶活性的影响。我们发现，射频场（RF）暴露 24 小时既不改变 RAS 和 ERK 激活的基础水平，也不改变佛波醇 12-肉豆蔻酸 13-乙酸酯（PMA）激活 RAS 和 ERK 激酶的效力。然而，我们发现，GSM 调制的 1800MHz 信号在 2W/kg 下的暴露降低了 PMA 激活 RAS 和 ERK 激酶活性的最大功效。CW 1800MHz 信号在 2W/kg 下的暴露仅降低了 PMA 激活 ERK 的最大功效，但不影响 RAS。无论使用何种信号，RF 暴露在 0.5W/kg 时均未观察到对 PMA 激活 RAS 或 ERK 的最大功效有任何影响。我们的结果表明，RF 暴露降低了从 PMA 与其受体结合到激活 RAS 和 ERK 激酶的一系列事件的效率。
