Cancer Biomark. 2017 Dec 12;21(1):73-80. doi: 10.3233/CBM-170273.
Accumulating evidences have shown that long non-coding RNAs (lncRNAs), longer than 200 nucleotides in length, play a crucial role in cancer occurrence and development. However, the relationships between most lncRNAs and gastric carcinogenesis remain poorly understood.
To explore the diagnostic value of one typical lncRNA, long intergenic non-protein coding RNA 1006 (LINC01006), in gastric cancer.
First, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to determine the expression levels of LINC01006 in various gastric tissues from gastric cancer patients, healthy controls, and gastric dysplasia. Next, the correlation between LINC01006 expression levels and clinicopathological features of patients with gastric cancer was assessed. Finally, the relative levels of LINC01006 in gastric cancer cell lines comparing to normal gastric epithelial cell line were analyzed.
LINC01006 levels in cancer tissues were significantly lower than those in adjacent normal tissues (P< 0.001) and healthy control tissues (P< 0.001). Its expression was associated with age (P= 0.013), tumor location (P= 0.022), tumor size (P= 0.030), and venous invasion (P= 0.018). Moreover, expression of LINC01006 was downregulated in two gastric cancer cell lines, MGC-803 (P< 0.05) and AGS (P< 0.001) compared to normal gastric epithelial cell line GES-1.
All the data implied that LINC01006 may be a novel biomarker for gastric cancer.
越来越多的证据表明，长度超过 200 个核苷酸的长非编码 RNA（lncRNA）在癌症的发生和发展中起着至关重要的作用。然而，大多数 lncRNA 与胃癌发生之间的关系仍知之甚少。
探讨一种典型的 lncRNA，长链非编码 RNA 1006（LINC01006）在胃癌中的诊断价值。
首先，采用实时定量逆转录聚合酶链反应（qRT-PCR）测定来自胃癌患者、健康对照者和胃黏膜上皮内瘤变患者的各种胃组织中 LINC01006 的表达水平。其次，评估 LINC01006 表达水平与胃癌患者临床病理特征之间的相关性。最后，分析胃癌细胞系与正常胃上皮细胞系相比 LINC01006 的相对水平。
癌组织中 LINC01006 的水平明显低于癌旁正常组织（P<0.001）和健康对照组织（P<0.001）。其表达与年龄（P=0.013）、肿瘤位置（P=0.022）、肿瘤大小（P=0.030）和静脉侵犯（P=0.018）有关。此外，与正常胃上皮细胞系 GES-1 相比，LINC01006 在两种胃癌细胞系 MGC-803（P<0.05）和 AGS（P<0.001）中的表达均下调。
所有数据表明，LINC01006 可能是胃癌的一种新型生物标志物。
