Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, Basel, Switzerland.
Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, Basel, Switzerland.
Trends Biotechnol. 2018 Jan;36(1):60-72. doi: 10.1016/j.tibtech.2017.10.003. Epub 2017 Oct 20.
Residing at the interface of chemistry and biotechnology, artificial metalloenzymes (ArMs) offer an attractive technology to combine the versatile reaction repertoire of transition metal catalysts with the exquisite catalytic features of enzymes. While earlier efforts in this field predominantly comprised studies in well-defined test-tube environments, a trend towards exploiting ArMs in more complex environments has recently emerged. Integration of these artificial biocatalysts in enzymatic cascades and using them in whole-cell biotransformations and in vivo opens up entirely novel prospects for both preparative chemistry and synthetic biology. We highlight selected recent developments with a particular focus on challenges and opportunities in the in vivo application of ArMs.
人工金属酶（ArMs）位于化学和生物技术的交界处，为将过渡金属催化剂的多功能反应库与酶的精确催化特性结合在一起提供了一种有吸引力的技术。虽然该领域早期的研究主要集中在明确的试管环境中，但最近出现了一种将 ArMs 应用于更复杂环境的趋势。将这些人工生物催化剂整合到酶级联中，并将其用于全细胞生物转化和体内，为制备化学和合成生物学开辟了全新的前景。我们重点介绍了最近的一些选定进展，特别关注 ArMs 体内应用的挑战和机遇。
