人工金属酶的设计和工程方面的最新进展。
Recent achievments in the design and engineering of artificial metalloenzymes.
机构信息
Departement of Chemistry, University of Basel, Spitalstrasse 51, Basel CH-4056, Switzerland.
Departement of Chemistry, University of Basel, Spitalstrasse 51, Basel CH-4056, Switzerland.
出版信息
Curr Opin Chem Biol. 2014 Apr;19:99-106. doi: 10.1016/j.cbpa.2014.01.018. Epub 2014 Mar 5.
Herein, we highlight a selection of recent successes in the creation of artificial metalloenzymes. A particular emphasis is set on different anchoring methods to incorporate the abiotic metal cofactor within the host protein as well as promising strategies for the de novo design of artificial metalloenzymes. Both approaches yield promiscuous catalytic activities which expand the catalytic repertoire of biocatalysis and synthetic biology. Moreover, we summarize laboratory evolution protocols which have contributed to unravel the full potential of artificial metalloenzymes.
在此，我们重点介绍了近期在人工金属酶创建方面取得的一些成功案例。特别强调了将非生物金属辅因子纳入宿主蛋白的不同锚定方法，以及人工金属酶从头设计的有前景的策略。这两种方法都产生了混杂的催化活性，从而扩展了生物催化和合成生物学的催化谱。此外，我们总结了有助于揭示人工金属酶全部潜力的实验室进化方案。
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