Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China; State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China.
Acta Biomater. 2018 Jan;65:163-173. doi: 10.1016/j.actbio.2017.10.031. Epub 2017 Oct 20.
A biomaterial combining fluorescent property with ability to induce osteogenesis can serve as an ideal multifunctional scaffold in bone tissue engineering. However, the frequently used fluorescent agents can only serve as imaging probes. The polymer or oligomer with a conjugated system containing nitrogen atoms will fulfill these criteria. In this study, a cyclo-trimer of acetonitrile is synthesized using a facile method, which is proved to be 4-amino-2,6-dimethylpyrimidine. The cyclo-trimer of acetonitrile demonstrates strong intrinsic photoluminescence and has the potential for in vivo imaging. The cyclo-trimer of acetonitrile shows no toxicity both in vitro and in vivo. Moreover, the cyclo-trimer of acetonitrile significantly promotes the osteogenesis of SaOS-2 cells by improving alkaline phosphatase activity, collagen type I and osteocalcin expression, as well as expressions of osteoblastic genes, and enhances the matrix mineralization of rBMSCs. Thus, the cyclo-trimer of acetonitrile synthesized in present study illustrates the employment of this kind multifunctional biomaterial in bone tissue engineering and may offer great potential in biomedical applications where bioimaging and osteogenesis are both required.
A conjugated cyclo-trimer of acetonitrile combining intrinsic fluorescent property with ability to induce osteogenesis was reported. Different from the traditional fluorescent dye or quantum dots, which are just "imaging agents", the cyclo-trimer of acetonitrile can serve as a multifunctional biomaterial and offer great potential in biomedical applications where bioimaging and osteogenesis are both required. To our best knowledge, the fluorescent property, especially fluorescent property in vivo and the ability of this molecule to induce osteogenesis have not been reported before. Our work illustrates the employment of this kind multifunctional biomaterial in bone tissue engineering and will highlight the importance of multifunctional biomaterial in biomedical applications.
一种兼具荧光性能和诱导成骨能力的生物材料可用作骨组织工程中的理想多功能支架。然而，常用的荧光剂只能作为成像探针。含有氮原子的共轭体系的聚合物或低聚物将满足这些标准。在这项研究中，我们采用一种简便的方法合成了一种乙腈的三聚体，它被证明是 4-氨基-2,6-二甲基嘧啶。乙腈三聚体具有很强的本征光致发光性能，具有体内成像的潜力。乙腈三聚体在体内和体外均无毒性。此外，乙腈三聚体通过提高碱性磷酸酶活性、I 型胶原和骨钙素的表达以及成骨基因的表达，促进 SaOS-2 细胞的成骨，并增强 rBMSCs 的基质矿化，从而显著促进成骨。因此，本研究合成的乙腈三聚体说明了这种多功能生物材料在骨组织工程中的应用，并可能在需要生物成像和成骨的生物医学应用中具有巨大的潜力。
本研究报道了一种兼具本征荧光性能和成骨诱导能力的共轭乙腈三聚体。与传统的荧光染料或量子点不同，它们只是“成像剂”，乙腈三聚体可用作多功能生物材料，在需要生物成像和成骨的生物医学应用中具有巨大的潜力。据我们所知，该分子的荧光性能，特别是体内荧光性能和成骨诱导能力以前没有报道过。我们的工作说明了这种多功能生物材料在骨组织工程中的应用，并强调了多功能生物材料在生物医学应用中的重要性。
