Nguyen Thi Duyen, Le Thi Mai Linh, Nguyen Huu Tien, Nguyen Thi Anh Duong, Liebanas Gracia, Trinh Quang Phap
Institute of Ecology and Biological Resource, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000 Hanoi, Vietnam.
Graduate University of Science and Technology, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000 Hanoi, Vietnam.
J Nematol. 2017 Sep;49(3):276-285.
sp. n. is described as associated with carrot ( subsp. sativus (Hoffm.) Schübl. & G. Martens) in Hai Duong Province, Vietnam. sp. n. is characterized by the lip region with three annuli and slightly separated from the body. Stylet knobs are rounded (never indented anteriorly). The lateral field includes four incisures, bearing areolation at the pharynx region and tail region and occasionally appears in the vulval region. Sometimes the appearances of oblique broken striaes divide the lateral field into five or six incisures. The ovary is distinct with one row of oocytes. Spermatheca is oval in shape with round central cavity, without sperm or reduced in some specimens. The postvuval uterine sac is long surpassing the vulva body diameter by 2 to 2.5 times (PUS = 31 to 65 µm). High vulva position with V = 66 to 75%. The tail shape can be subhemispherical with a smooth, slightly indented, broadly smooth, or cleft terminus observed in some specimens. The matrix code of sp. n. is: A2, B1, C4, D(1,3), E1, F(5,6), G(1,2), H(1,4); I(1,2,3,4), J1, K(1,2) according to Castillo and Vovlas (2007). The LSU-D2D3 segment and the ITS-rDNA region of this species were amplified and sequenced. The morphological characters and molecular phylogenetic analyses confirmed that this is a new species of the genus in Vietnam.
新物种被描述为与越南海阳省的胡萝卜（胡萝卜亚种（霍夫曼）舒布尔和G.马滕斯）相关。新物种的特征是唇区有三个环，与身体略有分离。口针瘤呈圆形（前部从不凹陷）。侧区包括四条侧线，在咽部区域和尾部区域有网纹，偶尔在外阴区域出现。有时斜向的断裂条纹会将侧区分成五或六条侧线。卵巢明显，有一排卵母细胞。受精囊呈椭圆形，中央腔圆形，有些标本中没有精子或精子减少。阴门后子宫囊很长，超过阴门体直径2至2.5倍（PUS = 31至65微米）。阴门位置高，V = 66至75%。尾部形状可以是近半球形，在一些标本中观察到末端光滑、略有凹陷、宽光滑或裂开。根据卡斯蒂略和沃夫拉斯（2007年），新物种的基质代码为：A2、B1、C4、D(1,3)、E1、F(5,6)、G(1,2)、H(1,4)；I(1,2,3,4)、J1、K(1,2)。对该物种的LSU-D2D3片段和ITS-rDNA区域进行了扩增和测序。形态特征和分子系统发育分析证实这是越南该属的一个新物种。
