Karaca Mehmet Sait, Yavuzaslanoglu Elif, Imriz Gul, Sonmezoglu Ozlem Ates
Department of Plant Protection, Ministry of Agriculture and Forestry, International Bahri Dagdas Agricultural Research Institute, Konya, Turkey.
Department of Plant and Animal Production, Vocational School, Karamanoglu Mehmetbey University, Karaman, Turkey.
J Nematol. 2020 Aug 25;52. doi: 10.21307/jofnem-2020-084. eCollection 2020.
(walnut root lesion nematode) is one of the most damaging root lesion nematode species worldwide. In this study, 17 populations of obtained from wheat and barley cultivated fields in 2016 to 2017 in Turkey (Karaman and Konya provinces) were identified using real-time PCR and melting curve analysis. Samples provided a single peak at 87.3˚C with real-time PCR. D2 to D3 expansion segments of the 28S rRNA of one population from Cihanbeyli district in Konya province was sequenced and recorded in GenBank (Accession number: MT320536.1). Alignments of the population was identical 98.66% to the populations of available in GenBank (Accs. nos: LT985479.1 and LT965052.1) and 98.65% (Accs. nos: KY424305.1 and KY424304.1).
（核桃根腐线虫）是全球最具危害性的根腐线虫种类之一。在本研究中，于2016年至2017年从土耳其（卡拉曼省和科尼亚省）的小麦和大麦种植田采集的17个群体，通过实时荧光定量PCR和熔解曲线分析进行了鉴定。实时荧光定量PCR检测样本在87.3˚C出现单峰。对科尼亚省锡汉贝利区一个群体的28S rRNA的D2至D3扩增片段进行了测序，并记录在GenBank中（登录号：MT320536.1）。该群体与GenBank中现有群体（登录号：LT985479.1和LT965052.1）的序列一致性为98.66%，与（登录号：KY424305.1和KY424304.1）的序列一致性为98.65%。
