Cheng Feixue, Wang Jian, Song Zhiqiang, Cheng Ju'e, Zhang Deyong, Liu Yong
Institute of Plant Protection, Hunan Academy of Agricultural Science, Changsha 410125, China.
Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province, Changsha 410125, China.
J Nematol. 2017 Sep;49(3):295-303.
Plant-parasitic nematodes are important agricultural pests and often cause serious crop losses. Novel, environmental friendly nematicides are urgently needed because of the harmful effects of some existing nematicides on human health. 5-Aminolevulinic acid (ALA) was reported as a potential biodegradable herbicide, insecticide, or plant-growth promoting agent. Lack of information on ALA against plant-parasitic nematodes prompted this investigation to determine the effects of ALA on , , , and . A series of in vitro assays and one greenhouse trial were conducted to examine the nematicidal effects of ALA. The results demonstrated that ALA exhibited a strong effect of suppression against the four nematodes tested. ALA also inhibited hatching of and . Results from the greenhouse experiment indicated that treatment of soil with 6.0 mM ALA significantly reduced the root-gall index (RGI) and egg mass number per root system compared with the uninoculated control ( ≤ 0.05). The metabolism assays indicated that ALA treatment significantly altered the nematode metabolism including the total protein production, malondialdehyde (MDA) content, and oxidase activities. This study suggested that ALA is a promising nematicide against plant-parasitic nematodes.
植物寄生线虫是重要的农业害虫，常常导致严重的作物损失。由于一些现有杀线虫剂对人类健康有害，因此迫切需要新型、环境友好型杀线虫剂。据报道，5-氨基乙酰丙酸（ALA）是一种潜在的可生物降解除草剂、杀虫剂或植物生长促进剂。由于缺乏关于ALA对植物寄生线虫作用的信息，开展了本研究以确定ALA对[此处原文中缺失具体线虫种类信息]、[此处原文中缺失具体线虫种类信息]、[此处原文中缺失具体线虫种类信息]和[此处原文中缺失具体线虫种类信息]的影响。进行了一系列体外试验和一项温室试验以检验ALA的杀线虫效果。结果表明，ALA对所测试的四种线虫表现出强烈的抑制作用。ALA还抑制了[此处原文中缺失具体线虫种类信息]和[此处原文中缺失具体线虫种类信息]的孵化。温室试验结果表明，与未接种对照相比，用6.0 mM ALA处理土壤显著降低了根瘤指数（RGI）和每个根系的卵块数量（P≤0.05）。代谢试验表明，ALA处理显著改变了线虫的代谢，包括总蛋白产量、丙二醛（MDA）含量和氧化酶活性。本研究表明，ALA是一种有前景的防治植物寄生线虫的杀线虫剂。
