Wang Dongwei, Wang Jian, Su Pin, Dai Jianping, Tan Xinqiu, Zhang Deyong, Liu Yong, Cheng Feixue
Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province, Institute of Plant Protection, Hunan Academy of Agriculture Sciences, Changsha, China.
Agricultural Economy and Regional Planning Research Institute, Hunan Academy of Agricultural Sciences, Changsha, China.
Front Microbiol. 2022 Sep 29;13:1021445. doi: 10.3389/fmicb.2022.1021445. eCollection 2022.
Root-knot nematode, is one of the most important nematodes affecting ginger crop. PSB-06, as effective microbial fertilizer in increasing plant growth and suppressing soil-borne disease of many crops has been reported. The combination of PSB-06 and dazomet treatments had been proved to inhibit root-knot nematode on ginger and increase ginger yield in our preliminary study. The field experiments were conducted to elucidate the reasons behind this finding, and followed by next-generation sequencing to determine the microbial population structures in ginger root rhizosphere. The results showed that combination of PSB-06 and dazomet treatment had a synergetic effect by achieving of 80.00% reduction in root-knot nematode numbers less than soil without treatment, and also could increase 37.37% of ginger yield through increasing the contents of chlorophyll and total protein in ginger leaves. Microbiota composition and alpha diversity varied with treatments and growth stages, soil bacterial diversity rapidly increased after planting ginger. In addition, the combined treatment could increase diversity and community composition of probiotic bacteria, and decrease those of soil-borne pathogenic fungi comparing to the soil treated with dazomet alone. Meanwhile, it could also effectively increase soil organic matter, available phosphorus and available potassium. Analysis of correlation between soil microorganisms and physicochemical properties indicated that the soil pH value and available phosphorus content were important factors that could affect soil microorganisms structure at the harvest stage. The bacterial family was more closely correlated with the soil physicochemical properties than the fungal family. Therefore, the combination of PSB-06 and dazomet was considered as an effective method to control root-knot nematode disease and improve ginger soil conditions.
根结线虫是影响生姜作物的最重要线虫之一。据报道，PSB - 06作为一种有效的微生物肥料，可促进多种作物生长并抑制土传病害。在我们的初步研究中，已证明PSB - 06与棉隆处理相结合可抑制生姜根结线虫并提高生姜产量。进行田间试验以阐明这一发现背后的原因，随后进行二代测序以确定生姜根际微生物种群结构。结果表明，PSB - 06与棉隆处理相结合具有协同效应，根结线虫数量比未处理土壤减少80.00%，还可通过增加生姜叶片中叶绿素和总蛋白含量使生姜产量提高37.37%。微生物群落组成和α多样性随处理和生长阶段而变化，种植生姜后土壤细菌多样性迅速增加。此外，与单独使用棉隆处理的土壤相比，联合处理可增加益生菌的多样性和群落组成，并减少土传致病真菌的多样性。同时，它还能有效增加土壤有机质、有效磷和有效钾。土壤微生物与理化性质的相关性分析表明，土壤pH值和有效磷含量是收获期影响土壤微生物结构的重要因素。细菌家族与土壤理化性质的相关性比真菌家族更密切。因此，PSB - 06与棉隆的组合被认为是控制根结线虫病和改善生姜土壤条件的有效方法。
