Mirajkar Akshata, Nikam Shashikant, Nikam Padmaja, Patil Giridhar
Department of Biochemistry, Belagavi Institute of Medical Sciences, Belgaum, Belagavi, Karnataka 590001 India.
Department of Medicine, Belagavi Institute of Medical Sciences, Belagavi, Karnataka 590001 India.
Indian J Clin Biochem. 2017 Oct;32(4):459-463. doi: 10.1007/s12291-016-0631-4. Epub 2016 Dec 28.
Human immunodeficiency virus (HIV) infection is a growing concern for health workers. The two major components of dyslipidemia in HIV infected patients are hypertriglyceridemia and decreased levels of high density lipoprotein (HDL) which contribute to increased atherosclerotic risk. The study included 150 newly diagnosed HIV patients and 150 healthy controls. In all these cases Lecithin cholesterol acyl transferase (LCAT) activity was assessed by measuring the difference between esterified and free cholesterol by digitonin precipitation method and levels of Apolipoprotein A-I (apo A-I) were measured by immunoturbidimetric method. There was a significantly decreased LCAT activity ( < 0.05) as well as reduced levels of apo A-I ( < 0.05) in newly diagnosed HIV patients (54.98 ± 3.44 IU/L and 131.85 ± 8.49 mg/dl) when compared with healthy controls (88.17 ± 14.36 IU/L and 187.05 ± 35.25 mg/dL). A significant positive correlation (r = 0.7406) was found between LCAT activity and apo A-I levels. In conclusion decreased LCAT activity and decreased levels of apo A-I reduce the reverse cholesterol transport in newly diagnosed HIV patients which may lead to atherosclerosis in the future.
人类免疫缺陷病毒（HIV）感染日益引起医护人员的关注。HIV感染患者血脂异常的两个主要组成部分是高甘油三酯血症和高密度脂蛋白（HDL）水平降低，这会增加动脉粥样硬化风险。该研究纳入了150例新诊断的HIV患者和150例健康对照。在所有这些病例中，通过用洋地黄皂苷沉淀法测量酯化胆固醇和游离胆固醇之间的差异来评估卵磷脂胆固醇酰基转移酶（LCAT）活性，并用免疫比浊法测量载脂蛋白A-I（apo A-I）水平。与健康对照（88.17±14.36 IU/L和187.05±35.25 mg/dL）相比，新诊断的HIV患者（54.98±3.44 IU/L和131.85±8.49 mg/dl）的LCAT活性显著降低（<0.05）以及apo A-I水平降低（<0.05）。LCAT活性与apo A-I水平之间存在显著正相关（r = 0.7406）。总之，LCAT活性降低和apo A-I水平降低会减少新诊断的HIV患者的胆固醇逆向转运，这可能在未来导致动脉粥样硬化。
