Haque Md Mazedul, Sarker Md Atiqur Rahman, Rifa Rafia Afroze, Islam Md Ariful, Khatun Mst Minara
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Vet World. 2017 Sep;10(9):1040-1045. doi: 10.14202/vetworld.2017.1040-1045. Epub 2017 Sep 11.
The present study was undertaken to determine bacterial load as well as characterize bacterial flora of ready to eat (RTE) betel leaf sold at local markets in Mymensingh city.
A total of 25 RTE betel leaf samples were collected from five local markets such as Kamal-Ranjit (KR) market, Shesh more, Kewatkhali, Jobber more, and Ganginar par.
Total viable count of bacteria in betel leaf (log mean colony forming unit±standard deviation/ml) was 7.58±0.04 for KR market, 7.72±0.06 for Shesh more, 7.62±0.04 for Kewatkhali, 7.40±0.03 for Jobber more, and 7.60±0.06 for Ganginar par. A total of 98 bacterial isolates belong to five genera (, spp., spp., spp., and spp.) were identified. The prevalence of was 17.34%, spp. was 25.51%, spp. was 19.39%, spp. was 18.37%, and spp. was 19.39%. Antibiotic sensitivity test showed that all isolates were sensitive to two antibiotics such as ciprofloxacin and gentamicin. Four isolates (, spp., spp., and spp.) were resistant to two antibiotics (ampicillin and cephalexin). Antibiogram profile of bacterial isolates of betel leaf suggests that they were multidrug resistance.
Data of this study indicate that betel leaf sold at local market harbors multidrug resistance food-borne bacteria which might cause public health hazards if these antibiotic resistant transfer to human through food chain.
本研究旨在确定孟加拉国米门辛希市当地市场上即食槟榔叶的细菌载量，并对其菌群进行特征分析。
从卡迈勒 - 兰吉特（KR）市场、谢什莫尔、凯瓦特卡利、乔伯莫尔和甘吉纳尔帕尔等五个当地市场共采集了25份即食槟榔叶样本。
KR市场槟榔叶中细菌的总活菌数（对数平均菌落形成单位±标准差/毫升）为7.58±0.04，谢什莫尔为7.72±0.06，凯瓦特卡利为7.62±0.04，乔伯莫尔为7.40±0.03，甘吉纳尔帕尔为7.60±0.06。共鉴定出98株属于五个属（、 spp.、 spp.、 spp.和 spp.）的细菌分离株。的流行率为17.34%， spp.为25.51%， spp.为19.39%， spp.为18.37%， spp.为19.39%。抗生素敏感性试验表明，所有分离株对环丙沙星和庆大霉素这两种抗生素敏感。有四株分离株（、 spp.、 spp.和 spp.）对氨苄西林和头孢氨苄这两种抗生素耐药。槟榔叶细菌分离株的抗菌谱表明它们具有多重耐药性。
本研究数据表明，当地市场销售的槟榔叶携带多重耐药食源细菌，如果这些抗生素耐药性通过食物链转移到人体，可能会对公众健康造成危害。
