Seifert Gabriel J, Sander Karoline C, Richter Sabine, Wittel Uwe A
Department of General and Visceral Surgery, Medical Center - University of Freiburg, Freiburg, Germany.
Ann Med Surg (Lond). 2017 Sep 28;24:8-14. doi: 10.1016/j.amsu.2017.09.012. eCollection 2017 Dec.
Little is known regarding the impact of host response in acute pancreatitis. Here, we induce murine necrotizing pancreatitis in 9 different mouse strains.
We examined 9 different mouse strains: Balb/CB4J, C3H/HEJ, NOD/SHILT, A/J, AKR/J, C57BI/6J, DBA/2J, FVB/NJ, 129S1/SvlmJ. 10 animals per strain were randomly allotted to two groups. Sterile necrotizing pancreatitis was induced by injection of taurocholate into the common bile duct. Control animals were injected with saline. Every 6 h, clinical parameters were examined and scored. After 24 h, animals were sacrificed to examine and compare serum enzymes, histology, bronchoalveolar lavage fluid, and serum IL-6.
Histologically, taurocholate treated animals scored significantly higher than control animals. Concordantly, serum lipase and amylase were significantly elevated in pancreatitis animals in all strains. NOD/SHILT and AKR/J mice had the highest enzyme activity. 24 h after induction, there were no signs of increased pulmonary vascular leak in taurocholate animals. Remarkably, interleukin 6 was not increased at all in C57BL/6J, C3H/HeJ, and 129S1/SvlmJ mice compared to all other strains.
The genetic strain has an impact on pancreatitis severity and systemic inflammatory response in a murine taurocholate induction model. Analogous differences in humans may partially account for the disparity in post-ERCP pancreatitis.
关于宿主反应在急性胰腺炎中的影响，人们了解甚少。在此，我们在9种不同的小鼠品系中诱导出鼠坏死性胰腺炎。
我们检测了9种不同的小鼠品系：Balb/CB4J、C3H/HEJ、NOD/SHILT、A/J、AKR/J、C57BI/6J、DBA/2J、FVB/NJ、129S1/SvlmJ。每个品系的10只动物被随机分为两组。通过向胆总管注射牛磺胆酸盐诱导无菌性坏死性胰腺炎。对照动物注射生理盐水。每6小时检查并记录临床参数。24小时后，处死动物以检测并比较血清酶、组织学、支气管肺泡灌洗液和血清白细胞介素-6。
组织学检查显示，牛磺胆酸盐处理的动物得分显著高于对照动物。相应地，所有品系的胰腺炎动物血清脂肪酶和淀粉酶均显著升高。NOD/SHILT和AKR/J小鼠的酶活性最高。诱导24小时后，牛磺胆酸盐处理的动物没有肺血管渗漏增加的迹象。值得注意的是，与所有其他品系相比，C57BL/6J、C3H/HeJ和129S1/SvlmJ小鼠的白细胞介素-6根本没有增加。
在鼠牛磺胆酸盐诱导模型中，基因品系对胰腺炎严重程度和全身炎症反应有影响。人类中的类似差异可能部分解释了内镜逆行胰胆管造影术后胰腺炎的差异。
