白细胞介素和CD14基因多态性与急性重症胰腺炎及感染性休克的关联。
Association of polymorphisms of IL and CD14 genes with acute severe pancreatitis and septic shock.
作者信息
Zhang Dian-Liang, Zheng Hong-Mei, Yu Bao-Jun, Jiang Zhi-Wei, Li Jie-Shou
机构信息
Department of General Surgery, Affiliated Hospital, Qingdao University Medial College, Qingdao 266003, Shandong Province, China.
出版信息
World J Gastroenterol. 2005 Jul 28;11(28):4409-13. doi: 10.3748/wjg.v11.i28.4409.
AIM
To investigate IL-1beta+3 594 in the 5th intron, IL-10-1 082 and CD14-159 polymorphisms in patients with acute pancreatitis (AP) and septic shock.
METHODS
The study included 215 patients (109 with acute severe pancreatitis (SAP), 106 with acute mild pancreatitis (MAP)) and 116 healthy volunteers. Genomic DNA was prepared from peripheral blood leukocytes. Genotypes and allele frequencies were determined in patients and healthy controls using restriction fragment length polymorphism analysis of PCR products.
RESULTS
The frequencies of IL-1beta+3 594T, IL-10-1082G and CD14-159T allele were similar in patients with mild or severe pancreatitis and in controls. Within SAP patients, no significant differences were found in the allele distribution examined when etiology was studied again. Patients with septic shock showed a significantly higher prevalence of IL-10-1082G allele than those without shock (chi2= 5.921, P = 0.015).
CONCLUSION
IL-10-1082G plays an important role in the susceptibility of SAP patients to septic shock. Genetic factors are not important in determination of disease severity or susceptibility to AP.
目的
研究急性胰腺炎（AP）合并感染性休克患者第5内含子中白细胞介素-1β（IL-1β）+3594、白细胞介素-10（IL-10）-1082和CD14-159基因多态性。
方法
本研究纳入215例患者（109例急性重症胰腺炎（SAP），106例急性轻症胰腺炎（MAP））和116名健康志愿者。从外周血白细胞中提取基因组DNA。采用聚合酶链反应（PCR）产物的限制性片段长度多态性分析，测定患者和健康对照的基因型及等位基因频率。
结果
轻症或重症胰腺炎患者与对照组中，IL-1β+3594T、IL-10-1082G和CD14-159T等位基因频率相似。在SAP患者中，再次研究病因时，所检测的等位基因分布无显著差异。感染性休克患者中IL-10-1082G等位基因的患病率显著高于无休克患者（χ2=5.921，P=0.015）。
结论
IL-10-1082G在SAP患者发生感染性休克的易感性中起重要作用。遗传因素在确定AP的疾病严重程度或易感性方面并不重要。
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