Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), P.O. Box 50, 6700 AB, Wageningen, the Netherlands.
Laboratorio de Microbiología y Biotecnología, Instituto Patagónico para el Estudio de los Ecosistemas Continentales (IPEEC, CONICET), Puerto Madryn, Argentina.
Microb Ecol. 2018 May;75(4):997-1008. doi: 10.1007/s00248-017-1091-y. Epub 2017 Oct 23.
Mangrove species are adapted to grow at specific zones in a tidal gradient. Here we tested the hypothesis that the archaeal and bacterial ammonia-oxidizing microbial communities differ in soils dominated by the mangrove species Avicennia germinans and Rhizophora mangle. Two of the sampling locations were tidal locations, while the other location was impounded. Differences in the community compositions of ammonia-oxidizing archaea (AOA) and bacteria (AOB) were analyzed by denaturing gradient gel electrophoresis (DGGE) of amoA genes and by MiSeq 16S rRNA gene-sequencing. The abundances of AOA and AOB were established by quantitative PCR of amoA genes. In addition, we analyzed the total microbial community composition based on 16S rRNA genes and explored the influence of soil physicochemical properties underneath Avicennia germinans and Rhizophora mangle on microbial communities. AOA were always more abundant than AOB, but the effect of mangrove species on total numbers of ammonia oxidizers was location-specific. The microbial communities including the ammonia oxidizers in soils associated with A. germinans and R. mangle differed only at the tidal locations. In conclusion, potential site-specific effects of mangrove species on soil microbial communities including those of the AOA and AOB are apparently overruled by the absence or presence of tide.
红树物种适应在潮汐梯度的特定区域生长。在这里，我们检验了以下假设：即在以红树物种海桑和桐花树为主的土壤中，古菌和细菌氨氧化微生物群落存在差异。两个采样地点是潮汐地点，而另一个地点是被围起来的。通过变性梯度凝胶电泳（DGGE）和 MiSeq 16S rRNA 基因测序分析氨氧化古菌（AOA）和细菌（AOB）的群落组成差异。通过定量 PCR 分析 amoA 基因确定 AOA 和 AOB 的丰度。此外，我们基于 16S rRNA 基因分析了总微生物群落组成，并探讨了海桑和桐花树下土壤理化性质对微生物群落的影响。AOA 的丰度总是高于 AOB，但红树物种对氨氧化菌总数的影响具有特定的位置特异性。与 A. germinans 和 R. mangle 相关的土壤中的微生物群落（包括氨氧化菌）仅在潮汐地点存在差异。总之，红树物种对包括 AOA 和 AOB 在内的土壤微生物群落的潜在特定地点的影响显然被潮汐的有无所掩盖。
