State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, China.
Laboratory of Basin Hydrology and Wetland Eco-restoration, China University of Geosciences, Wuhan, 430074, China.
Sci Rep. 2017 Mar 21;7:45028. doi: 10.1038/srep45028.
High abundance and widespread distribution of the archaeal phylum Bathyarchaeota in marine environment have been recognized recently, but knowledge about Bathyarchaeota in terrestrial settings and their correlation with environmental parameters is fairly limited. Here we reported the abundance of Bathyarchaeota members across different ecosystems and their correlation with environmental factors by constructing 16S rRNA clone libraries of peat from the Dajiuhu Peatland, coupling with bioinformatics analysis of 16S rRNA data available to date in NCBI database. In total, 1456 Bathyarchaeota sequences from 28 sites were subjected to UniFrac analysis based on phylogenetic distance and multivariate regression tree analysis of taxonomy. Both phylogenetic and taxon-based approaches showed that salinity, total organic carbon and temperature significantly influenced the distribution of Bathyarchaeota across different terrestrial habitats. By applying the ecological concept of 'indicator species', we identify 9 indicator groups among the 6 habitats with the most in the estuary sediments. Network analysis showed that members of Bathyarchaeota formed the "backbone" of archaeal community and often co-occurred with Methanomicrobia. These results suggest that Bathyarchaeota may play an important ecological role within archaeal communities via a potential symbiotic association with Methanomicrobia. Our results shed light on understanding of the biogeography, potential functions of Bathyarchaeota and environment conditions that influence Bathyarchaea distribution in terrestrial settings.
最近人们已经认识到古菌门 Bathyarchaeota 在海洋环境中的高丰度和广泛分布，但对于陆地环境中的 Bathyarchaeota 及其与环境参数的相关性的了解还相当有限。在这里，我们通过构建来自大九湖泥炭地的 16S rRNA 克隆文库，并结合对 NCBI 数据库中目前可用的 16S rRNA 数据的生物信息学分析，报道了不同生态系统中 Bathyarchaeota 成员的丰度及其与环境因子的相关性。总共对来自 28 个地点的 1456 个 Bathyarchaeota 序列进行了基于系统发育距离的 UniFrac 分析和基于分类的多元回归树分析。系统发育和基于分类的方法都表明，盐度、总有机碳和温度显著影响了 Bathyarchaeota 在不同陆地生境中的分布。通过应用“指示物种”的生态概念，我们在 6 个生境中确定了 9 个指示组，其中河口沉积物中最多。网络分析表明，Bathyarchaeota 成员形成了古菌群落的“骨干”，并经常与 Methanomicrobia 共同出现。这些结果表明，Bathyarchaeota 可能通过与 Methanomicrobia 的潜在共生关系在古菌群落中发挥重要的生态作用。我们的研究结果为理解 Bathyarchaeota 的生物地理学、潜在功能以及影响陆地环境中 Bathyarchaea 分布的环境条件提供了新的视角。
