Department of Andrology, Air Force General Hospital, PLA, Beijing, 100142, China.
Department of Chinese Medicine, Fangzhuang Community Health Service Center, Beijing, 100078, China.
Chin J Integr Med. 2019 May;25(5):342-347. doi: 10.1007/s11655-017-2425-9. Epub 2017 Oct 24.
To investigate the effect of a modified Wuzi Yanzong Pill (, WZYZP) on the male rats' testis after microwave radiation, as well as its potential mechanism.
Forty-five male rats were randomly assigned to three groups: the control group, the radiation group, and the WZYZP group. The rats in the radiation group and WZYZP group were exposed to microwave radiation for 15 min once, while the rats in the control group were not exposed to any radiation. The rats in the WZYZP group were given a modified of WZYZP by gavage daily for 7 days. Apoptosis in the testis was evaluated using terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) assay. Histopathological alterations of the testis were observed by haematoxylin-eosin (HE) staining. Tat-interactive protein, 60kD (Tip60) and p53 expressions were determined by Western blotting.
The apoptosis index (AI) in the radiation group was higher than that of the WZYZP group and control group on day 1 (D1), day 7 (D7) day 14 (D14) after radiation (P<0.05). The seminiferous tubules were of normal morphology in the control group. In the radiation group, the partial seminiferous tubules were collapsed, basement membranes of the seminiferous epithelia became detached. WZYZP could restore the morphological changes. There was no expression of Tip60 among the three groups on D7 and D14. The expression of p53 was higher in the radiation group than in the control group (P<0.05). WZYZP could down-regulate the rising p53 induced by radiation on D7 and D14 (P<0.05).
A modified WZYZP may affect germ cells, and its protective effects may partly result from its ability to intervene in Tip60 mediated apoptosis.
探讨五子衍宗丸（WZYZP）加味对微波辐射后雄性大鼠睾丸的影响及其作用机制。
45 只雄性大鼠随机分为 3 组：对照组、辐射组和 WZYZP 组。辐射组和 WZYZP 组大鼠接受 15 min 微波辐射 1 次，对照组大鼠不接受任何辐射。WZYZP 组大鼠每天灌胃给予 WZYZP 加味，共 7 天。采用末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）检测睾丸细胞凋亡，苏木精-伊红（HE）染色观察睾丸组织病理学改变，Western blot 法检测 Tat 相互作用蛋白 60kD（Tip60）和 p53 的表达。
辐射后 1、7、14 d，辐射组大鼠的凋亡指数（AI）高于 WZYZP 组和对照组（P<0.05）。对照组大鼠的生精小管形态正常，辐射组部分生精小管塌陷，生精上皮基底膜脱落，WZYZP 可恢复其形态改变。3 组大鼠在 D7、D14 时均无 Tip60 表达，辐射组大鼠 p53 表达高于对照组（P<0.05），WZYZP 可下调 D7、D14 时辐射诱导的 p53 升高（P<0.05）。
WZYZP 加味可能影响生殖细胞，其保护作用可能部分与抑制 Tip60 介导的细胞凋亡有关。
