五子衍宗方对环磷酰胺诱导的小鼠生殖细胞凋亡的保护作用

[Protective Effect of Wuzi Yanzong Prescription on Apoptosis in Germ Cells of Mice Induced by Cyclophosphamide].

作者信息

Huang Wei-feng, Zhang Chang-cheng, Liu Jing, Song Lai-xin, Peng Ben, Zhao Hai-xia

出版信息

Zhong Yao Cai. 2016 May;39(5):1143-7.

Abstract

OBJECTIVE

To investigate the protective effect and mechanism of Wuzi Yanzong prescription on apoptosis in germ cell of adult male mice induced by cyclophosphamide( CTX).

METHODS

Male Balb / C mice were randomly divided into normal control group,model group,the low-,medium- and high- dose of Wuzi Yanzong prescription groups( 100 mg / kg,200 mg / kg and 400 mg / kg),with 10 mice in each group. The mice were injected intraperitoneally with CTX( 200 mg / kg) from 4th day,and gave drug once a week,and executed for 4 weeks. Three doses of Wuzi Yanzong prescription were intragastrically administered every day. For normal control group,the same procedure was performed with intraperitoneal normal saline. Twelve hours after giving CTX at last time, all mice were weighed and sacrificed by cervical dislocation. The testis was immediately dissected and weighed, and then calculated the testis index. The pathological changes of testis were observed by HE staining,the apoptosis of germ cells were detected by TUNEL, the expression of apoptosis-related protein Caspase-3,BAX,BCL-2 in testis were examined by Western blot.

RESULTS

Compared with normal control group, the body weight, testis weight,testis index,and the expression of BCL-2 protein levels in testis of model control group were significantly decreased, the expression of BAX,Caspase-3 protein levels and apoptosis in testis of model control group were significantly increased. Wuzi Yanzong prescription significantly increased the body weight,testis weight,testis index,the expression of BCL-2 protein, while decreased the levels of BAX and Caspase-3 protein expression, and then led to the reduction in apoptosis of testis.

CONCLUSION

Wuzi Yanzong prescription can effectively protect the apoptosis of germ cell induced by CTX, and its mechanism may be associated with downregulating protein expression of BAX and Caspase-3,and increasing the protein expression of BCL-2.

摘要

目的

探讨五子衍宗方对环磷酰胺（CTX）诱导的成年雄性小鼠生殖细胞凋亡的保护作用及机制。

方法

将雄性Balb / C小鼠随机分为正常对照组、模型组、五子衍宗方低、中、高剂量组（100 mg / kg、200 mg / kg和400 mg / kg），每组10只。从第4天起腹腔注射CTX（200 mg / kg），每周给药1次，共4周。五子衍宗方三个剂量组每天灌胃给药。正常对照组腹腔注射生理盐水，操作同前。最后一次给予CTX 12小时后，称取所有小鼠体重，颈椎脱臼处死。立即解剖取出睾丸称重，计算睾丸指数。HE染色观察睾丸病理变化，TUNEL法检测生殖细胞凋亡，Western blot检测睾丸中凋亡相关蛋白Caspase-3、BAX、BCL-2的表达。

结果

与正常对照组相比，模型对照组小鼠体重、睾丸重量、睾丸指数及睾丸中BCL-2蛋白表达水平显著降低，模型对照组睾丸中BAX、Caspase-3蛋白表达水平及凋亡率显著升高。五子衍宗方可显著增加小鼠体重、睾丸重量、睾丸指数及BCL-2蛋白表达，同时降低BAX和Caspase-3蛋白表达水平，进而导致睾丸细胞凋亡减少。