State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Institute of Qinghai Tibetan Plateau, Southwest Minzu University, Chengdu, China.
Pharm Biol. 2022 Dec;60(1):1533-1541. doi: 10.1080/13880209.2022.2101670.
Wuzi Yanzong Prescription (WYP) has long been used to treat male infertility, with convincing clinical evidence. However, its mechanism of action is not clear.
WYP chemical components were quantified by HPLC, and the effect on oligoasthenozoospermia rats was explored based on UPLC-Q-TOF-MS metabonomics technology.
The solution was extracted by alcohol and water; the content of eight components in the extracting solution was determined by HPLC. Twenty-four Sprague-Dawley rats were randomly divided into control (CG), model (MG) and administration (PG) groups. Oligoasthenozoospermia was induced for 30 days in all, but the CG with daily oral doses of 20 mg/kg glycosides (TG). PG was given daily oral doses of WYP solution (1.08 g/kg), CG and MG received the same volume of distilled water, all administered for 30 days. After the last administration, the serum samples were collected and detected by UPLC-Q-TOF-MS.
An HPLC method for simultaneous determination of chlorogenic acid (0.081%), ellagic acid (0.021%), hyperoside (0.032%), verbascoside (0.011%), isoquercitrin (0.041%), astragalin (0.026%), kaempferol (0.009%) and schisandrin (0.014%) was established. Moreover, the 74 potential biomarkers and eight metabolic pathways related to oligoasthenozoospermia were identified by multivariate analysis and metabolite profiling. WYP can regulate 36 markers, mainly involving amino acid metabolism, lipid metabolism and nucleotide metabolism.
This is a simple and accurate method for quality control of WYP. It further enriches the potential mechanism of WYP in the treatment of oligoasthenozoospermia. It can provide a theoretical basis for the rational application of WYP.
五子衍宗方（WYP）长期以来一直用于治疗男性不育症，具有令人信服的临床证据。然而，其作用机制尚不清楚。
采用高效液相色谱法（HPLC）对 WYP 的化学成分进行定量，并基于 UPLC-Q-TOF-MS 代谢组学技术探讨其对少弱精子症大鼠的作用。
采用醇-水提取法提取溶液；采用 HPLC 法测定提取液中 8 种成分的含量。将 24 只 Sprague-Dawley 大鼠随机分为对照组（CG）、模型组（MG）和给药组（PG）。所有大鼠均连续 30 天诱导少弱精子症，但 CG 每日口服 20mg/kg 糖苷（TG）。PG 每日口服 WYP 溶液（1.08g/kg），CG 和 MG 给予等体积蒸馏水，均给药 30 天。末次给药后，采集血清样本，采用 UPLC-Q-TOF-MS 进行检测。
建立了同时测定绿原酸（0.081%）、鞣花酸（0.021%）、金丝桃苷（0.032%）、毛蕊花糖苷（0.011%）、异槲皮苷（0.041%）、芦丁（0.026%）、山奈酚（0.009%）和五味子醇甲（0.014%）的 HPLC 方法。此外，通过多元分析和代谢物谱图分析，鉴定出与少弱精子症相关的 74 个潜在生物标志物和 8 条代谢途径。WYP 可调节 36 个标志物，主要涉及氨基酸代谢、脂质代谢和核苷酸代谢。
这是一种简单、准确的 WYP 质量控制方法。它进一步丰富了 WYP 治疗少弱精子症的潜在机制。可为 WYP 的合理应用提供理论依据。
